Detecting Single Seeds of Small Broomrape (<i>Orobanche minor</i>) with a Polymerase Chain Reaction

Fig. 5. Multiplex PCR demonstrating the specificity of the small broomrape-specific primers (377 bp band) and the amplification of the internal control primers (555 bp band). Orobanche minor from Multnomah (lanes 1 and 2), Clackamas (lane 4), and Columbia (lane 5) counties; O. californica (lane 3); O. fasciculata (lane 6); water control (lane 7); and 100 bp DNA ladder (L).



Image from Plant Health Progress article:
Detecting Single Seeds of Small Broomrape (Orobanche minor) with a Polymerase Chain Reaction