Detecting Single Seeds of Small Broomrape (<i>Orobanche minor</i>) with a Polymerase Chain Reaction

Fig. 6. Multiplex PCR detection of a single small broomrape seed (377 bp band) and the internal control (555 bp band) in the presence of red clover DNA. DNA from one (lane 1), five (lane 2), ten (lane 3), and twenty (lane 4) seeds of small broomrape combined with red clover DNA; red clover and small broomrape positive controls combined (lane 5); small broomrape positive control alone (lane 6); red clover positive control alone (lane 7); water control (lane 8); and 100 bp DNA ladder (L).



Image from Plant Health Progress article:
Detecting Single Seeds of Small Broomrape (Orobanche minor) with a Polymerase Chain Reaction