Detecting <i>Orobanche minor</i> Seeds in Soil Using PCR

Fig. 2. Multiplex PCR detection of seeds of small broomrape (377 bp amplicon) and the internal control (555 bp amplicon) extracted from soil with a 2.25 M CaCl2 or a 9 M CaCl2 solution. DNA from soil with 0 (lanes 1 and 2), 5 (lanes 3 and 4), and 10 (lanes 5 and 6) seeds added; small broomrape positive control (lane 7); water control (lane 8); 100 bp DNA ladder (lane L). The 2.25 M CaCl2 solution was used on the samples shown in the odd-numbered lanes and the 9 M CaCl2 solution used on samples shown in the even-numbered lanes.

Image from Plant Health Progress article:
Detecting Orobanche minor Seeds in Soil Using PCR