First Report of <i>Tomato spotted wilt virus</i> in Hosta in Florida

Fig. 2. Detection of Tomato spotted wilt virus (TSWV) infection in hosta plants by reverse transcription-polymerase chain reaction (RT-PCR). Total RNA was extracted from leaves of non-symptomatic (NS; Lane 3) and symptomatic (S; Lanes 4 and 5) hosta plants, amplified by RT-PCR with virus specific primers, analyzed by native electrophoresis on a 2% agarose gel and stained with ethidium bromide. A positive control (+; Lane 2) consisted of total RNA extracted from leaves of a Key West nightshade plant (Solanum bahamense) mechanically inoculated with TSWV. Lane 1 (M) contains markers with sizes in kilobases (kb) indicated to the left of the gel.

Image from Plant Health Progress article:
First Report of Tomato spotted wilt virus in Hosta in Florida