First Report of <i>Hibiscus latent Fort Pierce virus</i> in New Mexico

Fig. 4. Detection of Hibsicus latent Fort Pierce virus (HLFPV) by immunocapture reverse-transcription polymerase chain reaction (IC-RT-PCR). Samples of uninfected Nicotiana tabacum leaves (lane 1), asymptomatic leaves of Hibiscus rosa-sinensis (lanes 2 and 3) and H. syriacus (lane 4), and a symptomatic leaf of H. syriacus were tested for HLFPV infection by IC-RT-PCR with specific primers, analyzed by gel electrophoresis in a 1% agarose gel and imaged with a Kodak Image Station 2000. Comparison with DNA markers included on the gel (not shown) was used to determine the size in base pairs of the PCR products. All asymptomatic leaves (lanes 2-4) tested negative for HLFPV by tissue blot immunoassay, whereas symptomatic leaves (lane 5) tested positive.



Image from Plant Health Progress article:
First Report of Hibiscus latent Fort Pierce virus in New Mexico