<i>Phytophthora hibernalis</i>: A New Pathogen on <i>Rhododendron</i> and Evidence of Cross Amplification with Two PCR Detection Assays for <i>Phytophthora ramorum</i>

Fig. 4. Amplicons from the second amplification reaction of the nested PCR protocol for the detection of Phytophthora ramorum separated on a 3% Nusieve 3:1 agarose gel. Lane 1, size standards; Lane 2, negative control, without DNA; Lane 3, DNA extract of P. ramorum culture; Lanes 4 to 9, DNA extracts of P. hibernalis-inoculated rhododendron. Extracts of even numbered lanes from the first amplification reaction are diluted 1:10 and odd numbered lanes 1:100 before adding to the second amplification reaction. Lanes 4 and 5, 6 and 7, and 8 and 9 represent three individual inoculated leaves.



Image from Plant Health Progress article:
Phytophthora hibernalis: A New Pathogen on Rhododendron and Evidence of Cross Amplification with Two PCR Detection Assays for Phytophthora ramorum