Early Detection of Asian Soybean Rust Using PCR

Fig. 1. Agarose gel of conventional PCR performed at the University of Tennessee using DNA extracted from soybean leaves infected with Phakopsora pachyrhizi and the ASR-specific primers. The first lane contains a low mass DNA ladder (Invitrogen, Carlsbad, CA) with fragment sizes of 100, 200, 400, 600, 1200, and 2000 bp. Sample numbers correspond to the number of days post infection (dpi), and the letters indicate inoculum spore concentrations: A = 12,500/ml; B = 25,000/ml; C = 50,000/ml; and D = 100,000/ml. The arrow indicates the first clearly visible PCR amplification product.



Image from Plant Health Progress article:
Early Detection of Asian Soybean Rust Using PCR