Occurrence of Viroids in Commercial Hop (<i>Humulus lupulus</i> L.) Production Areas of Washington State

Fig. 1. Amplification products from reverse transcription-polymerase chain reactions (RT-PCR) were analyzed to detect viroids in hop leaves. Duplex RT-PCR was used to detect Hop latent viroid (HLVd) and Hop stunt viroid (HSVd) (lanes 1 to 6). Lanes: 1, water control; 2, healthy hop seedling; 3, ‘Tettnanger’ hop plant infected with HLVd; 4, ‘Golden Light Peach’ from Korea (positive control for HSVd); 5, ‘Glacier’ hop plant infected with HLVd; and 6, ‘Glacier’ hop plant infected with HLVd and HSVd. A separate RT-PCR was used to detect Apple fruit crinkle viroid (AFCVd) (lanes 7 to 11). Lanes: 7, water control; 8, healthy hop seedling; 9, pear sample infected with the apscaviroid Pear blister canker viroid; 10, ‘Glacier’ hop plant infected with HLVd; and 11, ‘Glacier’ hop plant infected with HLVd and HSVd. The size in base pairs of molecular size markers (MW) are indicated on the left.



Image from Plant Health Progress article:
Occurrence of Viroids in Commercial Hop (Humulus lupulus L.) Production Areas of Washington State