Isolation, Storage, Pathotype Characterization, and Evaluation of Resistance for <i>Phytophthora sojae</i> in Soybean

Fig. 1B. Isolation of P. sojae from plant tissues: (A) wash in 0.5% sodium hypochlorite solution; (B, shown here) blot dry; (C) place tissue on PBNIC medium; (D) flip the agar so the symptomatic tissue is sandwiched between the plate and the agar; (E) after flipping, cut the agar from the edge of the plate and seal around each piece to promote contact with agar and bottom of the plate; and (F) check plates at 4 to 5 days to observe typical growth pattern of mycelium on PBNIC agar.



Image from Plant Health Progress article:
Isolation, Storage, Pathotype Characterization, and Evaluation of Resistance for Phytophthora sojae in Soybean