Factors to Improve Detection of <i>Alfalfa mosaic virus</i> in Soybean

Fig. 2. ELISA readings of trifoliolate leaf samples from soybean cultivars Lee68 (A-D) and Colfax (E) rub-inoculated with Alfalfa mosaic virus strains (A) H4, (B) S40, (C) W1, and (D, E) S30 are shown. The inoculated plants were maintained in a growth chamber at 25°C with a photoperiod of 16 h. Leaflets at each node that were present at the time of sampling were sampled 30, 60, and 90 days postinoculation (dpi). The samples at 30 dpi consisted of the middle leaflet of each of the existing trifoliolate leaves while the left and then right leaflet of each of the existing trifoliolate leaves were sampled at 60- and 90-dpi, respectively. Schematic positions of the trifoliolate leaves sampled for analysis by ELISA are depicted at the right side of the panels.



Image from Plant Health Progress article:
Factors to Improve Detection of Alfalfa mosaic virus in Soybean