Real-time PCR Detection of <i>Rhodococcus fascians</i> and Discovery of New Plants Associated with <i>R. fascians</i> in Pennsylvania

Fig. 1. Sensitivity of detection of Rhodococcus fascians using extracted DNA and bacterial cells. (A) Standard curve of cycle threshold (Ct) values calculated from 10-fold serial dilutions of DNA (150 ng/µl) from R. fascians strain Rf-1. Three separate real-time PCR amplifications using a combination of the Rf-229F/Rf-408R primer pair and probe Rf-366P were performed for each dilution. The straight line was calculated by linear regression (Microsoft Excel).



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Real-time PCR Detection of Rhodococcus fascians and Discovery of New Plants Associated with R. fascians in Pennsylvania