Identification of <i>Tobacco streak virus</i> Associated With a Virus-like Mottle Symptom on <i>Hosta</i>

Fig. 2. PCR detection of TSV from cDNAs synthesized from immunocaptured virions (Lanes 1, 2) and dsRNA template (Lanes 3, 4) with MP-specific primers (Lanes 1, 3) and CP-specific primers (Lanes 2, 4). Water controls with MP primers (Lane 5), CP primers (Lane 6), and Hosta virus X (HVX) triple gene block primers (TGB) (Lane 7). HVX TGB clone used as a positive PCR control with HVX TGB primers (Lane 8). M = 1 Kb DNA ladder (250, 500, 750, 1000, 1500, and 2000 bp markers indicated). Electrophoresis was done in 0.8% agarose at 100 volts for 90 min in 1X TAE buffer. Gel was stained with ethidium bromide. MP and CP amplicons are 1040 bp and 950 bp, respectively.



Image from Plant Health Progress article:
Identification of Tobacco streak virus Associated With a Virus-like Mottle Symptom on Hosta