Identification of Two <i>Tobacco rattle virus</i> Variants Associated with Line Pattern Disease of Bleeding Heart in Ohio

Fig. 3. PCR detection of TRV from cDNAs synthesized from dsRNA template from symptomatic (Lanes 1, 2) and asymptomatic (Lanes 3, 4) bleeding heart with Tobravirus group (Lanes 1, 3) and TRV specific (Lanes 2, 4) primers. Water controls with Tobravirus group (Lane 5) and TRV specific (Lane 6) primers, and TRV positive control with Tobravirus group (Lane 7) and TRV specific (Lane 8) primers. M = 1 Kb DNA ladder (250, 500, 750, 1000 bp markers indicated). Electrophoresis was done in 0.8% agarose at 100 volts for 60 min in 1X TAE buffer.



Image from Plant Health Progress article:
Identification of Two Tobacco rattle virus Variants Associated with Line Pattern Disease of Bleeding Heart in Ohio