Downy Mildew from Lake Erie Vineyards is Diverse for the G143A SNP Conferring Resistance to Quinone Outside Inhibitor Fungicides

Fig. 2. Gel demonstrating the CAPS-PCR RFLP method of SNP determination. (A) Gel image of initial PCR of collected samples showing the 651 bp band amplified from the P. viticola cytochrome b gene. Lanes are marked with sample identification number. C denotes reaction run with DNA extracted from uninfected Vitis hybrid ‘Concord’ leaves demonstrating the primers do not amplify any plant genes. (B) Same samples as the above following digest with the restriction enzyme Fnu4HI (syn. Ita1). Sample 117 shows the characteristic pattern of a sample that contains the G143A SNP. The small band is a combination of the 322 bp and 329 bp bands that are the result of the digest. The presence of two bands is the result of a mixed wild type and mutant population on the leaf.



Image from Plant Health Progress article:
Downy Mildew from Lake Erie Vineyards is Diverse for the G143A SNP Conferring Resistance to Quinone Outside Inhibitor Fungicides