Identification, Transmission, and Partial Characterization of a Previously Undescribed Flexivirus Causing a Mosaic Disease of Ash (<i>Fraxinus</i> spp.) in the USA

Fig. 2. (A) Characterization of white ash mosaic virus (WAMV) virions. Virion morphology in a purified preparation negatively-stained with aqueous uranyl formate. Scale bar equals 200 nm. (B) Electrophoretic migration pattern under denaturing conditions of genomic ssRNA extracted from purified WAMV virions. Electrophoresis was conducted in 1.5% agarose in Tris-acetate-EDTA. (C) Estimation of WAMV coat protein (CP) subunit molecular mass by SDS-PAGE. Lane 1, BenchMark 10-200 kDa protein ladder (Invitrogen). Lane 2, WAMV coat protein.



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Identification, Transmission, and Partial Characterization of a Previously Undescribed Flexivirus Causing a Mosaic Disease of Ash (Fraxinus spp.) in the USA