Identification of Two <i>Tobacco streak virus</i> Capsid Protein Variants Associated With Leaf Mottle and Necrosis Symptoms on <i>Astilbe</i>

Fig. 2. PCR detection of TSV from cDNAs synthesized from dsRNA template with MP-specific primers (Lane 1) and CP-specific primers (Lane 2). Water controls with MP primers (Lane 3) and CP primers (Lane 4). TSV-Hosta MP clone with MP primers (Lane 5) and TSV-Hosta CP clone with CP primers (Lane 6) used as positive PCR controls. M=1 Kb DNA ladder (250, 500, 750, 1000, and 1500 bp markers indicated). Electrophoresis was performed in 0.8% agarose at 100 volts for 60 min in 1X TAE buffer. Gel was stained with ethidium bromide. MP and CP amplicons are 1036 bp and 947 bp, respectively.



Image from Plant Health Progress article:
Identification of Two Tobacco streak virus Capsid Protein Variants Associated With Leaf Mottle and Necrosis Symptoms on Astilbe