Identification of <i>Poinsettia mosaic virus</i> Associated with a Virus-like Mottle Symptom on Poinsettia in Ohio

Fig. 2. PCR amplification of PnMV ORF 1 regions from cDNAs synthesized from dsRNA template with degenerate TYMOfwd290 + rev1073 and TYMOfwd4381 + rev4759 (Lanes 1 and 2, respectively) tymovirus and PnMVfwd264 + rev1098 and PnMVfwd1023 + rev2195 (Lanes 3 and 4, respectively) PnMV primers. Water controls with TYMOfwd290 + rev1073, TYMOfwd4381 + rev4759 (Lanes 5 and 6, respectively), PnMVfwd264 + rev1098, PnMVfwd1023 + rev2195 (Lanes 7 and 8, respectively), and Tobacco rattle virus (TRV) specific primers (Lane 9). A TRV clone with TRV specific primers was used as a positive PCR control (Lane 10) due to identical reaction and cycling conditions to those used for the PnMV and degenerate tymovirus primers. M = 1 Kb DNA ladder (250, 500, 750, 1000, 1500 bp markers indicated). Electrophoresis was performed in 0.8% agarose at 100 volts for 60 min in 1X TAE buffer.



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Identification of Poinsettia mosaic virus Associated with a Virus-like Mottle Symptom on Poinsettia in Ohio