Identification of a Previously Undescribed Satellite RNA Associated with a <i>Cucumber mosaic virus</i> Subgroup II Strain from <i>Pratia pedunculata</i> in Ohio

Fig. 1. PCR detection of CMV from cDNAs synthesized from immunocaptured virions from Pratia pedunculata with MP-specific (Lane 1), CP-specific (Lane 2), and satRNA-specific (Lane 3) primers. A CMV isolate from Vinca minor in Nicotiana tabacum ‘Glurk’ with MP-specific (Lane 4), CP-specific (Lane 5), and satRNA-specific (Lane 6) primers used as a positive IC/RT control. Water negative controls with MP (Lane 7), CP (Lane 8), and satRNA (Lane 9) primers. CMV-Vinca satRNA clone (Lane 10) used as a positive PCR control with satRNA-specific primers. M = 1 Kb DNA ladder (250, 500, 750, 1000, and 1500 bp markers indicated). Electrophoresis was performed in 0.8% agarose at 100 volts for 60 min in 1X TAE buffer. Gel was stained with ethidium bromide. MP, CP, and satRNA amplicons are 1194, 974, and 339 nt, respectively.



Image from Plant Health Progress article:
Identification of a Previously Undescribed Satellite RNA Associated with a Cucumber mosaic virus Subgroup II Strain from Pratia pedunculata in Ohio