Identification of <i>Lily mottle virus</i> and <i>Lily symptomless virus</i> Co-infecting Asiatic Lily in Ohio

Fig. 2. PCR detection of LMoV from cDNAs synthesized from immunocaptured virions with TBV/LMoVfwd540+rev1370 (Lane 1) and TBV/LMoVfwd900+rev2005 (Lane 2) primers. Water controls with TBV/LMoVfwd540+rev1370 (Lane 3), TBV/LMoVfwd900+rev2005 (Lane 4), LSV-RdRp (Lane 5), and LSV-CP (Lane 6) primers. LSV clones with LSV-RdRp (Lane 7) and CP (Lane 8) primers used as positive PCR controls because of compatible reaction and cycling conditions. M = 1 Kb DNA ladder (250, 500, 750, 1000, and 1500 bp markers indicated). Electrophoresis was performed in 0.8% agarose at 100 volts for 60 min in 1X TAE buffer. Gel was stained with ethidium bromide. LMoV amplicons are 829 bp and 1102 bp, respectively.

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Identification of Lily mottle virus and Lily symptomless virus Co-infecting Asiatic Lily in Ohio