Organizing Committee


Poster Presentations


Poster Presentations


Assessment of genetic variation among Asian soybean rust isolates using microsatellites

Presenter: S. J. Anderson

All authors and affiliations: S. J. ANDERSON (1), C. L. Stone (1), M. Lucia Posada-Buitrago (2), J. L. Boore (2), B. A. Neelam (3), R. M. Stephens (3), D. G. Luster (1), K. F. Pedley (1), and R. D. Frederick (1). (1) USDA-ARS, Foreign Disease-Weed Science Research Unit, Fort Detrick, MD 21702; (2) DOE Joint Genome Institute, Walnut Creek, CA 94598; and (3) Advanced Biomedical Computing Center, NCI-Frederick, Frederick, MD

Asian soybean rust, caused by Phakopsora pachyrhizi, is a devastating foliar disease of soybeans that has spread from Asia to Africa, South America, and the United States. Repeatmasker and StrFinder programs were used to mine microsatellites from P. pachyrhizi genomic sequences deposited in GenBank. Twenty-eight P. pachyrhizi isolates were surveyed using 24 microsatellite markers. Nineteen of these microsatellite markers were useful for assessing genetic variation among the isolates. Six P. pachyrhizi isolates collected from Louisiana and Alabama in November 2004 were genetically diverse. These six U.S. isolates also showed different reaction phenotypes when inoculated onto soybean accessions containing the four rust resistance genes Rpp1-Rpp4.

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