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9

Proteome analysis of greenhouse-grown soybean plants inoculated with Phakopsora pachyrhizi

Presenter: Z.-Y. Chen

All authors and affiliations: Z.-Y. CHEN, S. Park, N. Hazard, E. P. Mumma, and R. W. Schneider. Louisiana State University Agricultural Center, Baton Rouge, LA 70803

Phakopsora pachyrhizi, the causal agent of Asian soybean rust, was first reported in Japan in 1902, and later it was found in China and other Asian countries. In recent years, this disease entered Africa and South America and has spread rapidly since. Now, it is an emerging disease in the continental United States since its discovery in late 2004. Upon infection, the affected plants are quickly defoliated, resulting in reduced yields by as much as 80% and in significant economic losses to soybean growers. Currently, no commercial variety has resistance to this disease and it can be controlled to some extent through costly fungicide applications. The objective of the present study is to identify host and fungal proteins induced during infection using proteomics to understand host-fungus interactions. Soybeans were planted in the greenhouse and were inoculated at the R1 stage with P. pachyrhizi urediniospores that were previously collected from field-infected soybean plants. Leaves were harvested from infected and noninfected plants 10 days after inoculation. Total leaf proteins were extracted and separated using large format 2-D gels. The unique and upregulated proteins in the rust-infected soybean leaves were identified through Progenesis 2-D gel analysis software.

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