Organizing Committee


Poster Presentations


Poster Presentations


Comparison of 2005 and 2006 Phakopsora pachyrhizi spore rain trap data

Presenter: C. W. Barnes

All authors and affiliations: C. W. BARNES (1,2), L. J. Szabo (1,2), V. C. Bowersox (3), and K. S. Harlin (3). (1) Cereal Disease Laboratory, USDA-ARS, St. Paul, MN; (2) University of Minnesota, St. Paul, MN; and (3) NADP, Illinois State Water Survey, Champaign, IL

In 2005 and 2006, rain was collected weekly at more than 100 selected National Atmospheric Deposition Program (NADP) sites across the eastern and central United States, from North Dakota to Texas and from Florida to New York. Rain samples were filtered and screened for Phakopsora pachyrhizi (Asian soybean rust fungal) DNA using a nested real-time PCR assay. The assay was used to screen more than 1,500 samples collected from May to August in both 2005 and 2006. The temporal distribution was similar between the years, with the number of positive samples increasing through May and early June, decreasing in mid to late June and into July, and increasing again through August. However, there was a threefold increase in the number of samples testing positive for P. pachyrhizi in 2006. In addition, the results indicate that there was an increase in spore load in 2006 compared to 2005. This year, P. pachyrhizi DNA was detected in every state in the study region except for North Dakota.

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