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18

A monoclonal-based lateral flow device for the early and reliable field detection of Phakopsora pachyrhizi

Presenter: German Hoyos(1)

Other authors and affiliations: Brendan Gow(1), Joan Lawton(1), Karen Larkin(1), Terry Goddard(1), Gilberto Olaya(2). (1)EnviroLogix Inc., Research and Development, Portland, ME 04102, U.S.A.; (2)Syngenta Crop Protection, Vero Beach, FL, U.S.A.

Asian soybean rust (ASBR), caused by the fungus Phakopsora pachyrhizi, may result in soybean yield losses of up to 80%. Accurate and timely diagnosis of ASBR is the key to determining whether a response will be attempted and, if so, the extent, direction, and magnitude of that response. To date, a reliable way to diagnose ASBR in the field is the identification of the small (2- to 5-mm), tan to dark brown, sporulating lesions that occur days after infection. Attempts to identify the disease before sporulation are difficult since symptoms could be confused with other common soybean diseases. A polyclonal (Paby)-based lateral flow device (LFD) developed by EnviroLogix in 2006 assisted in the diagnosis of this disease in the field prior to development of mature pustules. EnviroLogix has now developed an improved second generation LFD that incorporates a monoclonal antibody (Maby). The major improvement with this new LFD is significantly increased positive signal intensities across the detection range (early noncharacteristic visual symptoms through mature SBR symptoms).

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