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2

Proteomic analysis of soybean accessions resistant and susceptible to soybean rust (Phakopsora pachyrhizi)

Presenter: S. Park

All authors and affiliations: Z. Chen (1), S. PARK (1), M. C. Ganiger (1), A. A. Fortunato (1). (1) Louisiana State University, Baton Rouge, LA

Asian soybean rust, caused by the fungus Phakopsora pachyrhizi, is a menace to soybean growers worldwide. This disease was discovered in the continental United States in late 2004 and has the potential to cause severe yield reduction and billions of dollars in economic losses because all U.S. commercial soybean varieties are susceptible to this disease. In an effort to understand compatible and incompatible host-pathogen interactions at the molecular level, 16 accessions were evaluated with rust spores collected in Louisiana. Two accessions showed consistent immune response. qRT-PCR was conducted to compare the progress of fungal proliferation in resistant and susceptible lines. Fungal biomass was significantly increased 2 days after infection in susceptible lines, whereas no or little increase was detected in resistant lines. Protein profiles of two resistant and two susceptible lines were compared to identify proteins differentially expressed between resistant and susceptible lines with or without rust infection. Differentially expressed proteins were observed in both resistant and susceptible lines. Some of them matched with previously identified proteins, such as pathogenesis-related protein 10, S-adenosylmethionine synthetase I, and 3´5´-cyclic nucleotide phosphodiesterase. The identities of other differentially expressed proteins are being determined through peptide sequencing. The potential importance of these differentially expressed proteins in soybean-P. pachyrhizi interaction will be discussed.

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