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6

Transcriptome analysis of Phakopsora pachyrhizi-Glycine max interaction in susceptible plant

Presenter: A. Tremblay

All authors and affiliations: A. TREMBLAY (1), P. Hosseini (1), N. W. Alkharouf (2), B. F. Matthews (1). (1) USDA-ARS-Plant Sciences Institute, Beltsville, MD; (2) Towson University, Towson, MD

Protection of soybean from new exotic pathogens is very important for soybean production. Soybean rust is caused by the obligate parasitic fungus Phakopsora pachyrhizi Sydow. We analyzed the expression pattern of P. pachyrhizi genes and genes of its soybean host during the infection process. Thus, we constructed and analyzed the cDNA library to identify candidate genes that may be useful to provide resistance to P. pachyrhizi. The library was sequenced using a Solexa/Illumina platform. We obtained 4,723,211 sequences that align on the soybean genome. Of these, numerous sequences didn’t have any annotation. From sequences without similarity (1,467,084) to the soybean genome, contigs were formed and homology searches were conducted to determine the identity of the genes. These are expected to be P. pachyrhizi sequences. Six percent of these contigs had homology with proteins in the NCBI database. Although a number of hits described hypothetical or predicted proteins, we found interesting genes coding for interesting proteins, such as the negative regulator of differentiation 1 involved in fungal development as well as a chitin deacetylase, a glyoxal oxidase involved in lignin degradation, different proteins involved in signal transduction (serine threonine protein phosphatase, calcineurin subunit b), and a transthyretin protein involved in intracellular communication. Some of these target pathogen and some host genes will be studied to determine if they can be used to control Asian soybean rust in soybean.

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