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Real-time PCR Detection of Rhodococcus fascians and Discovery of New Plants Associated with R. fascians in Pennsylvania

E. V. Nikolaeva, S. Kang, T. N. Olson, and S. H. Kim

February 2012


Rhodococcus fascians is a gram-positive bacterium that causes bacterial fasciation on a wide range of ornamental plants. To address the need for a reliable, sensitive, and specific method for detecting R. fascians in infected plant materials, a real-time (RT) PCR assay was developed. The objectives of this study were to develop a reliable RT PCR assay to detect pathogenic R. fascians in clinical samples and to document new plants associated with R. fascians in Pennsylvania greenhouses.


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