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Development and Evaluation of AmplifyRP Acceler8 Diagnostic Assay for the Detection of Fusarium oxysporum f. sp. vasinfectum Race 4 in Cotton

H. K. Doan, S. Zhang, and R. M. Davis

March 2014


A rapid and reliable molecular diagnostic assay, AmplifyRP Acceler8, was developed for the direct detection of Fusarium oxysporum f. sp. vasinfectum (FOV) race 4, a virulent genotype of the Fusarium wilt pathogen of cotton, in soil and cotton tissue. The amplification is carried out at a single constant temperature without the need of a thermal cycler.


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