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A Microfluidic Assay for Identifying Differential Responses of Plant and Human Fungal Pathogens to Tobacco Phylloplanins

C. Mattupalli, J. E. Spraker, E. Berthier, A. O. Charkowski, N. P. Keller, and R. W. Shepherd

August 2014

Research

Phylloplanins are defensive glycoproteins secreted onto leaf surfaces by trichome-bearing plants such as tobacco. They are of interest because of their antimicrobial properties, but like other natural product bioactives, the assessment and screening of phylloplanins biological activity is impeded by limited availabilities of active compounds. Here, we report an inexpensive microfluidic approach that requires ≤ 20 microliters of tobacco phylloplanins to assess spore germination inhibition of plant and human fungal pathogens.

doi:10.1094/PHP-RS-14-0009

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