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Factors to Improve Detection of Alfalfa mosaic virus in Soybean

B. He, J. H. Hill, and M. R. Hajimorad

September 2011


Infection of plants with Alfalfa mosaic virus is known to be associated with symptom and virus concentration remission, a phenomenon known as "recovery." However, for the routine detection of AMV in the infected plants, this phenomena is problematic. Indeed in soybean, detection of AMV has been problematic and inconsistent results have been obtained from tissues collected at different growth stage of the infected plants. In order to determine optimum sampling procedure for analysis by ELISA, three soybean cultivars and five biologically distinct strains of AMV were utilized in this study. Each cultivar was rub-inoculated with each of the strains and all the trifoliolate leaf samples of the infected plants were analyzed by ELISA at 30, 60, and 90 days postinoculation. Results suggested that for all virus strains and cultivars tested, tissue samples should consist of pooled fully developed trifoliolate leaflets that are positioned at the upper parts of the plants. Additionally, symptoms of AMV infection were dependent upon soybean cultivar and virus strain and varied from symptomatic to asymptomatic. This makes it problematic to conclude a soybean plant is AMV infected based upon symptomatology alone.


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