Home

Presentations

Author Index

Second International Research Conference on Huanglongbing - 2011

Proceedings
Sponsor

University of Florida
Institute of Food &
Agricultural Sciences
Citrus Research &
Education Center

Conference
Organizers

University of Florida
Institute of Food &
Agricultural Sciences
Citrus Research &
Education Center

U. S. Horticultural Research Laboratory

Florida Department
of Agriculture &
Consumer Services

Conference
Sponsors

PDF of
complete list


Proceedings of the Conference

Orlando, Florida January 10-14, 2011


Download entire symposium as a one 5-MB file in PDF format,

or download individual elements as smaller PDFs, below.

Title Page

Editors, Committees, and Copyright 1
Sponsors and Organizers 2
Index of Presentations as PDF (see also below) 4
 
No. Presentation and Authors Page

 

Session 1: Pathogen Genomics, Bioinformatics, Phylogenetics, and Culturing − John Hartung, Moderator

1.1

Genomic Comparisons of the Ca. Liberibacter asiaticus Chromosome with Other Members of the Rhizobiales
Hartung, J.S., Shao, J., Kuykendall, L.D.

17

1.2

Genetic and Functional Characterization of the znu Operon in the Intracellular Citrus Pathogen, Candidatus Liberibacter asiaticus
Vahling, C.M., Benyon, L.S., Duan, Y.-P.

18

1.3

Comparison of the Ca. Liberibacter asiaticus Genome with a Draft Ca. L. americanus Genome Reveals Similar Prophage with Likely Pathogenicity Factors
Zhang, S.J., Wulff, N.A., Flores-Cruz, Z., Zhou, L.J., Kang, B.-K., Fleites, L.A., Gooch, M.D., Davis, M.J., Duan, Y.-P., Gabriel, D.W.

19

1.4

Analysis of Candidatus Liberibacter americanus Genome
Wulff, N.A., Zhang, S.J., Ayres, A.J., Bové, J.M., Gabriel, D.W.

20

1.5

Population Genetics Analysis of Candidatus Liberibacter asiaticus from Multiple Continents
Glynn, J.M., Bai, Y., Chen, C., Duan, Y.-P., Civerolo, E.L., Lin, H.

21

1.6

Phylogenetic Analysis of Asian Candidatus Liberibacter asiaticus; Asian Common Strains Are Distributed in Northeast India, Papua New Guinea, and Timor-Leste
Miyata, S., Kato, H., Tomimura, K., Davis, R., Smith, M.W., Weinert, M., Iwanami, T.

22

1.7

Bioinformatic Analysis of Genome Sequence Data for Ca. Liberibacter asiaticus
Lindeberg, M., Saha, S.

23

1.8

Genetic Diversity of Candidatus Liberibacter asiaticus Isolates from Paraná State, Brazil
Meneguim, L., Marques, V.V., Murata, M.M., Barreto, T.P., Vasquez-Souza, G.V., Villas-Boas, L.A., Paccola-Meirelles, L.D., Leite, R.P., Jr.

24

1.9

Analysis of Endophytic Bacterial Diversity from Huanglongbing Pathogen-Infected Citrus Tissues
Wang, A., Yin, Y., Li, Y., Li, J., Xian, J., Wang, Z.

30

1.10

Evolving Diversity of Candidatus Liberibacter asiaticus Revealed by Comparative Analysis of Two Intragenic Tandem Repeat Genes
Zhou, L.J., Powell, C.A., Hoffman, M., Li, W.B., Fan, G.-C., Liu, B., Duan, Y.-P.

31

1.11

In vitro Culture of the Fastidious Bacteria Candidatus Liberibacter asiaticus in Association with Insect Feeder Cells
Fontaine-Bodin, L., Fabre, S., Gatineau, F., Dollet, M.

32

1.12

Preliminary Report of Cultivation of Candidatus Liberibacter asiaticus from Citrus Tissue with Huanglongbing
Xie, P., Yin, Y., Li, Y., Li, J., Wang, Z.

33

1.13

Characterization of Highly Mosaic Genomic Loci of Candidatus Liberibacter asiaticus in Southern China and Florida
Wang, X.F., Zhou, C.Y., Deng, X., Su, H.N., Chen, J.


34

1.14

Further Evidence That U.S. and Chinese Populations of Candidatus Liberibacter asiaticus are Different
Deng, X., Liu, R., Zhang, P., Chen, J.
    

38
Session 2: Asian Citrus Psyllid Biology and Genomics − David Hall, Moderator

2.1

Phylogeographic and Population Genetic Studies Uncover Two Founding Events in Asian Citrus Psyllid Populations Collected in the Americas
de León, J.H., Sétamou, M., Gastaminza, G.A., Buenahora, J., Cáceres, S., Yamamoto, P.T., Logarzo, G.A., Stañgret, C.R.W.

40

2.2

Alteration of Microbiome of Bactericera cockerelli and Diaphorina citri Based on Candidatus Liberibacter sp. Infection
Hail, D., Hunter, W.B., Bextine, B.R.

41

2.3

Oral Uptake of dsRNA Increases Mortality in Diet Fed Psyllids
Shatters, R.G., Jr., Powell, C.A., Borovsky, D.

42

2.4

The Psyllid Feeding Process: Composition and Biosynthetic Inhibition of the Salivary Sheath
Shatters, R.G., Jr.

43

2.5

A New Method for Short-Term Rearing of Psyllid Adults and Nymphs on Detached Citrus Leaves and Young Terminal Shoots
Ammar, E.-D., Hall, D.G.

44

2.6

Comparative Analysis of Asian Citrus Psyllid and Potato Psyllid Antennae
Arras, J., Hunter, W.B., Bextine, B.R.

45

2.7

The Emerging Psyllid Genome: RNA-Interference and Insect Biology
Hunter, W.B., Bextine, B.R., Shatters, R.G., Jr., Reese, J., Shelby, K.S., Hall, D.G.

46

2.8

Bacterial Population Diversity in Diaphorina citri: Analysis by PCR- DGGE and RFLP Methodology
Wang, Z., Tian, S., Liu, T., Yin, Y.
   

47
Session 3: Asian Citrus Psyllid Ecology and Transmission − Lukasz Stelinski, Moderator

3.1

Antennal Responses of Diaphorina citri to Host Plant Volatiles Recorded Using a Coupled Gas Chromatograph Electroantennogram Detector System
Robbins, P.S., Alessandro, R.T., Lapointe, S.L.

49

3.2

Population Dynamics of the Asian Citrus Psyllid and Potential Generations in Northern Sinaloa, Mexico
Cortez-Mondaca, E., López- Arroyo, J.I., Pérez-Márquez, J., González, V.M.

50

3.3

Localization of Candidatus Liberibacter asiaticus in Dissected Organs of Its Psyllid Vector Diaphorina citri Using Fluorescent in situ Hybridization and Quantitative PCR
Ammar, E.-D., Shatters, R.G., Jr., Hall, D.G.

51

3.4

Interactions of the Asian Citrus Psyllid, Diaphorina citri, with Candidatus Liberibacter asiaticus
Pelz-Stelinski, K.S., Rogers, M.E.


52

3.5

Seasonal Changes in Numbers of Asian Citrus Psyllids Carrying Candidatus Liberibacter asiaticus
Ebert, T.A., Brlansky, R.H., Rogers, M.E.

53

3.6

Understanding Diaphorina citri-Candidatus Liberibacter asiaticus Interactions and D. citri Behavior for Managing Huanglongbing (HLB) in Florida
Mann, R.S., Pelz-Stelinski, K.S., Rouseff, R.L., Stelinski, L.L.

54

3.7

Effects of Soil-Applied and Foliar-Applied Insecticides on Asian Citrus Psyllid (Diaphorina citri) Feeding Behavior and Their Possible Implication for HLB Transmission
Serikawa, R.H., Okuma, D.M., Backus, E.A., Rogers, M.E.

55

3.8

Effect of Insecticides and Mineral Oil on Probing Behavior of Diaphorina citri Kuwayama (Hemiptera: Psyllidae) in Citrus
de Miranda, M.P., Felippe, M.R., Garcia, R.B., Yamamoto, P.T., Lopes, J.R.S.

56

3.9

A New Detached-Leaf Assay Method to Test the Inoculativity of Psyllids with Candidatus Liberibacter asiaticus Associated with Huanglongbing Disease
Ammar, E.-D., Walter, A., Hall, D.G.

57

3.10

Preliminary Study of Comparative Acquisition of Candidatus Liberibacter asiaticus and Ca. L. americanus by Diaphorina citri Under Different Temperatures
Barbosa, J.C., Eckstein, B., Belasque, J., Jr., Bergamin Filho, A.


59

3.11

Host Range of Diaphorina citri Kuwayama and Leuronota fagarae on Citrus and Zanthoxylum spp.
Russell, D.N., Halbert, S.E., Roberts, P.D.

60

3.12

Abundance of Diaphorina citri (Hemiptera: Psyllidae) in Orange Jasmine and Backyard Citrus of Yucatán, Mexico
Lozano-Contreras, M., Jasso-Argumedo, J., Morales-Koyoc, D., Jasso-Laucirica, T., González-Hernández, A., López-Arroyo, J.I.

61

3.13

Difference of Gender and Effect of Photoperiod on Asian Citrus Psyllid Feeding Behavior
Okuma, D.M., Serikawa, R.H., Rogers, M.E.

62

3.14

Seasonal Abundance of Diaphorina citri (Hemiptera: Psyllidae) and Natural Enemies in Citrus Groves of Yucatán, Mexico
Jasso-Argumedo, J., Lozano-Contreras, M., Barroso-Aké, H., López-Arroyo, J.I.

63

3.15

Host Plants of Psyllids in South Texas
Thomas, D.B.
    

64
Session 4: Survey, Detection and Diagnosis − John da Graça, Moderator

4.1

Development and Reactivity of Polyclonal Antibodies Based on OMP Sequences of Candidatus Liberibacter asiaticus
Coletta-Filho, H.D., Peroni, L.A., De Souza, A.A., Takita, M.A., Stach-Machado, D.R.

67

4.2

Development of Single-Chain Antibody Fragments (scFVs) Against Candidatus Liberibacter asiaticus by Phage Display
Yuan, Q., Jordan, R., Brlansky, R.H., Minenkova, O., Hartung, J.S.

68

4.3

Highly Sensitive Detection by Real-Time PCR Targeting the Multiple Tandem Repeats of Two Prophage Region Genes of the Citrus Huanglongbing Disease Bacterium, Candidatus Liberibacter asiaticus
Morgan, J.K., Zhou, L.J., Shatters, R.G., Jr., Manjunath, K.L., Duan, Y.-P.

69

4.4

Comparison of Different Extraction and Assay Protocols in Different Laboratories to Develop a Standardized Assay for Detection of Huanglongbing-Associated Bacteria from Psyllids
Manjunath, K.L., Irey, M.S., Ramadugu, C., Lee, R.F., Levesque, C.S., Brady, B., Polek, M.L., Lin, H., Civerolo, E.L., Afunian, M., Vidalakis, G.

70

4.5

Assessment of Various Spectroscopic Techniques for Detection of HLB
Poole, G.H., Hawkins, S.A., Windham, W.R., Heitschmidt, J., Albano, J.P., Park, B., Lawrence, K.C., Gottwald, T.R.

71

4.6

Seasonal Variability in HLB Testing Data in Plant and Psyllid Samples in Florida
Irey, M.S., Gast, T., Cote, J., Gadea, P., Santiago, O., Briefman, L., Graham, J.H.

72

4.7

Survey to Estimate the Rate of HLB Infection in Florida Citrus Groves
Irey, M.S., Morris, R.A., Estes, M.

73

4.8

Two Survey Protocols to Detect Newly Introduced HLB and Other Exotic Pathogens and Pests
Gottwald, T.R., Riley, T.D., Irey, M.S., Parnell, S.R., Hall, D.G.

74

4.9

Distribution of Candidatus Liberibacter Americanus and Ca. L. asiaticus in Foliage of Naturally Infected Citrus Trees
Sousa, M.C., Lemos, M.V.F., Frare, G.F., Santos, M.A., Lopes, S.A.

75

4.10

A Perspective on the Activities of Texas HLB Diagnostic Laboratory
Kunta, M., da Graça, J.V., Sétamou, M., Skaria, M.

76

4.11

Two New Real-Time PCR-Based Surveillance Systems for Candidatus Liberibacter Species Detection
Lin, H., Bai, Y., Civerolo, E.L.


77

4.12

Detection of Candidatus Liberibacter solanacearum in Potato Psyllid Isolated from Sticky Traps
Kwok, K., Levesque, C.S., Manjunath, K.L., Irey, M.S., Polek, M.L.

78

4.13

Detection of Candidatus Liberibacter asiaticus (Las) on Yellow Sticky Traps by Real-Time PCR
Irey, M.S., Gadea, P., Hall, D.G.

80

4.14

Validation of the Starch-Iodine Reaction for Field Pre-Diagnosis of Huanglongbing in Citrus of México
Loredo-Salazar, R.X., Uribe- Bustamante, A., Rodríguez-Quibrera, C.G., Curtí-Díaz, S.A., Alanís- Martínez, E.I., Velázquez-Monreal, J.J., López-Arroyo, J.I.

81

4.15

Detecting HLB Using NIR Remote Sensing
Gonzalez-Mora, J., Dima, C.S., Irey, M.S., Ehsani, R.

82

4.16

Isothermal Detection of Huanglongbing in Psyllids and Citrus Tree Samples
Russell, P.F., McGowen, N., Bohannon, R.

83

4.17

Assessment of Candidatus Liberibacter asiaticus in the Psyllids, Diaphorina citri Collected from Murraya paniculata in Thailand
Jantasorn, A., Duan, Y.-P., Hoffman, M., Zhang, S., Puttamuk, T., Thaveechai, N.


87

4.18

Liberibacter Reservoirs in Cities and Villages in the State of São Paulo, Brazil
Lopes, S.A., Frare, G.F., Camargo, L.E.A., Wulff, N.A., Teixeira, D.C., Bassanezi, R.B., Beattie, G.A.C., Ayres, A.J.

88

4.19

Pictorial Gallery of Foliar HLB Symptoms on Various Citrus Varieties and Citrus Relatives
Robl, D.J., Riley, T.D., Gomez, H.
     

89
Session 5: Economics, Fruit Quality, and Crop Loss − Mike Irey, Moderator

5.1

Evaluation of Chemical Flavor Compounds in Orange Juice from Multiple Harvests of Hamlin and Valencia Fruit from HLB- Symptomatic Versus Healthy Trees
Baldwin, E., Bai, J., Dea, S., Plotto, A., Manthey, J., Rouseff, R.L., Irey, M.S.

91

5.2

Evaluation of Bitterness Caused by Huanglongbing Disease in Orange Juice
Dea, S., Plotto, A., Manthey, J., Baldwin, E., Irey, M.S.

92

5.3

Sensory Evaluation of Juice Made with Fruit from Huanglongbing (HLB) Affected Trees
Plotto, A., Valim, F., Rouseff, R.L., Dea, S., Manthey, J., Narciso, J., Bai, J., Irey, M.S., Baldwin, E.

93

5.4

Economic Considerations to Treating HLB with the Standard Protocol or an Enhanced Foliar Nutritional Program
Morris, R.A., Muraro, R.P.

94

5.5

When Should a Grower with HLB Stop Removing Trees?
Irey, M.S.

95

5.6

Use of Electronic Sensor Technology to Discriminate between Juices from Huanglongbing Infected and Healthy Orange Trees
Bai, J., Dea, S., Plotto, A., Baldwin, E., Irey, M.S.

96

5.7

A Regional Epidemiological Approach for Yield Loss Estimates Due to Candidatus Liberibacter Under Different Risk Scenarios
Mora-Aguilera, G., Acevedo, G., López-Arroyo, J.I., Velázquez-Monreal, J.J., Gómez, R., Robles-González, M.M., Salcedo, D.
     

97
Session 6: Epidemiology − Tim Gottwald, Moderator

6.1

Five Years of Experience with Huanglongbing in Florida: Current Assessment; How Did We Get Here?
Halbert, S.E., Manjunath, K.L., Ramadugu, C., Lee, R.F.

99

6.2

Designing Sampling Schemes to Maximize the Probability of Early Detection of a Huanglongbing Outbreak
Parnell, S.R., Gottwald, T.R., van den Bosch, F.

100

6.3

Candidatus Liberibacter africanus Subspecies capensis on Calodendrum capense in South Africa
Phahladira, M.N.B., Viljoen, R., Pietersen, G.

101

6.4

Distribution of Psyllids Positive for Candidatus Liberibacter asiaticus in Citrus Groves in Southwest Florida
Halbert, S.E., Manjunath, K.L., Ramadugu, C., Mears, P., Lee, R.F.

102

6.5

Seasonal Prevalence of Citrus Huanglongbing (Candidatus Liberibacter asiaticus) in a Central Florida Sweet Orange Grove
Parkunan, V., Wang, N.-Y., Ebert, T.A., Rogers, M.E., Dewdney, M.M.

103

6.6

A Mathematical Model for Transmission of HLB by Psyllids
Chiyaka, C., Singer, B., Halbert, S.E., van Bruggen, A.H.C.

104

6.7

Potential Spread of Huanglongbing Through Soil
Nunes da Rocha, U., Dickstein, E.R., van Bruggen, A.H.C.
     

105
Session 7: International Citrus Industries, Regulation, and Grower Experiences − MaryLou Polek, Moderator

7.1

Laws, Huanglongbing Management, and the Current Status of the Disease in São Paulo, Brazil
Belasque, J., Jr., Ayres, A.J., Barbosa, J.C., Massari, C.A., Bové, J.M.

107

7.2

Distribution of Citrus Huanglongbing in the Dominican Republic
Matos, L., Hilf, M.E., Cayetano, X., Feliz, A., Puello, H., Méndez, F., Borbón, J., Folimonova, S.Y.

108

7.3

Citrus Huanglongbing in Cuba: Current Situation, Management, and Main Research
López, D., Luis, M., Collazo, C., Batista, L., Peña, I., González, C., Pérez, J.L., Zamora, V., Borroto, A., Pérez, D., Alonso, E., Acosta, I., Llauger, R., Casín, J.C.

109

7.4

Spreading and Symptoms of Huanglongbing in Mexican Lime Groves in the State of Colima, Mexico
Robles-González, M.M., Velázquez-Monreal, J.J., Manzanilla Ramirez, M.A., Orozco Santos, M., Floina Urrutia, V.M., Carrillo Medrano, S.H.

110

7.5

The Asian Citrus Psyllid/Huanglongbing Detection, Treatment, and Regulatory Program in California
Galindo, T.

111

7.6

Detection and Reporting of Asian Citrus Psyllid and Huanglongbing in Commercial Citrus Within California: An Industry Program
Taylor, B.J., Polek, M.L., Batkin, T.

112

7.7

Citrus Health Research Forum: A National Research Effort
Polek, M.L., Wisler, G.

113

7.8

The Identification and Distribution of Citrus Greening Disease in Jamaica
Oberheim, A.P., Brown, S.E., McLaughlin, W.A.

114

7.9

Fitting a Spatial Analysis Grid for Research on Huanglongbing in Mexico
Aldama-Aguilera, C., Olvera-Vargas, L.A., Galindo-Mendoza, M.G.
     

115
Session 8: Host-Pathogen Interactions − Bill Dawson, Moderator

8.1

Examination of Stages of the HLB Disease Development in Citrus Trees
Folimonova, S.Y., Achor, D.S., Hilf, M.E.

117

8.2

New Defense Response Insights of Sweet Orange Infected with Two Candidatus Liberibacter Species
Mafra, V.S., Martins, P.K., Locali- Fabris, E.C., Ribeiro-Alves, M., Francisco, C.S., Freitas-Astúa, J., Kishi, L.T., Machado, M.A.

118

8.3

Differential Expression of Potential Virulence Genes of Candidatus Liberibacter asiaticus in Infected Plants and Psyllids
Sreedharan, A., Wei, S., Wang, N.-Y.

119

8.4

Metabolome Analysis of Tolerant and Susceptible Citrus Varieties in Response to Infection with Candidatus Liberibacter asiaticus
Albrecht, U., Skogerson, K., Bowman, K.D., Fiehn, O.

120

8.5

Deep Transcriptome Profiling of Citrus Fruit in Response to Huanglongbing Disease
Martinelli, F., Uratsu, S.L., Albrecht, U., Reagan, R.L., Leicht, E., D’Souza, R., Bowman, K.D., Dandekar, A.M.

124

8.6

Carbohydrate Metabolism and Related Gene Expression Changes in Huanglongbing-Affected Sweet Orange
Chen, C., Fan, J., Yu, Q., Brlansky, R.H., Li, Z.-G., Gmitter, F.G., Jr.

125

8.7

Analysis of Colonization of Citrus Seeds by Ca. Liberibacter asiaticus and Its Possible Role in Seed Transmission
Hilf, M.E.

126

8.8

Natural Transmission of Huanglongbing Caused by Candidatus Liberibacter americanus and Ca. L. asiaticus and with Two Different Sources of Inoculum Plants (Citrus sinensis or Murraya exotica)
Gasparoto, M.C.G., Bassanezi, R.B., Amorim, L., Montesino, L.H., Lourenço, S.A., Wulff, N.A., Bergamin Filho, A.

127

8.9

Callose Predominates over Phloem Protein 2 in Phloem Plugging of Trees Affected with Huanglongbing
Albrigo, L.G., Achor, D.S.

128

8.10

Influence of Huanglongbing (HLB) on the Composition of Citrus Juices and Mature Leaves
Cancalon, P.F., Bryan, C., Haun, C., Zhang, J.

129

8.11

Gene Expression in Citrus sinensis Fruit Tissues Harvested from Huanglongbing-Infected Trees
Liao, H.-L., Burns, J.K.

131

8.12

Expression Profiling of Host Response of Citrus to Candidatus Liberibacter asiaticus Infection
Aritua, V., Wang, N.-Y.

132

8.13

Arabidopsis Responses to the HLB-Relative Candidatus Liberibacter psyllaurous
Patne, S., Manjunath, K.L., Roose, M.L.

133

8.14

Comparative Studies of the Endophytic Microbial Community Structures in Huanglongbing-Infected and Non-Infected Citrus Plants
Zheng X.-F., Liu, B., Ruan, C.-Q., Lin, Y.-Z., Xiao, R.-F., Zhu, Y.-J., Fan, G.-C., Cai, Z.J., Duan, Y.-P.

134

8.15

HLB Influences the Diversity, Structure, and Function of the Bacterial Community Associated with Citrus
Trivedi, P., Wang, N.-Y.

135

8.16

Functional Studies of Putative Effectors of Candidatus Liberibacter asiaticus Using Citrus Tristeza Virus Vector
Hajeri, S., Duan, Y.-P., Gowda, S.

136

8.17

First Report of a New Host (Pithecellobium lucidum Benth) of the Citrus Huanglongbing Bacterium, Candidatus Liberibacter asiaticus
Fan, G.-C., Cai, Z.J., Weng, Q.Y., Ke, C., Liu, B., Zhou, L.J., Duan, Y.-P.

137

8.18

Citrus Seed Grafting: A Simple Technology for Testing Seed Transmission of Citrus Greening/HLB and of Other Pathogenic Agents
Bar-Joseph, M., Robertson, C., Hilf, M.E., Dawson, W.O.

138

8.19

Lack of Transmission of HLB by Citrus Seed
Graham, J.H., Johnson, E.G., Bright, D.B., Irey, M.S.

139

8.20

Visualization of Ca. Liberibacter asiaticus in Immature Citrus Seed Coats by Fluorescent In Situ Hybridization (FISH) of 16S rRNA
Hilf, M.E.

140

8.21

Rapid, Sensitive, and Non-Radioactive Tissue-Blot Diagnostic Method for the Detection of Citrus Greening Disease (HLB)
Gowda, S., Nageswara Rao, N., Miyata, S., Ghosh, D.K., Irey, M.S., Rogers, M.E., Garnsey, S.M.
      

141
Session 9: Asian Citrus Psyllid Management − Michael Rogers, Moderator

9.1

A Database for Analysis of Diaphorina citri Population Monitoring Data from Commercial Groves
Gast, T., Irey, M.S., Hou, H.

143

9.2

RNAi Strategy in Citrus Trees to Reduce Hemipteran Pests: Psyllids and Leafhoppers
Hunter, W.B., Glick, E., Bextine, B.R., Paldi, N.

148

9.3

Application of Insecticidal Sprays to Citrus in Winter Provides Significant Reduction in Asian Citrus Psyllid Diaphorina citri Populations and Opportunity for Additional Suppression Through Conservative and Augmentative Biological Control
Qureshi, J.A., Stansly, P.A.

149

9.4

Studies on Imidacloprid and Management of ACP in California
Byrne, F., Morse, J.G., Bethke, J.


150

9.5

Selection and Dosage of Insecticides for the Control of the Asian Citrus Psyllid in the Citrus Groves of Mexico
López-Arroyo, J.I., Díaz- Zorrilla, U., Hernández-Fuentes, L.M., Cortez-Mondaca, E., Robles- González, M.M., Villanueva-Jiménez, J.A., Cabrera-Mireles, H., Loera- Gallardo, J., Jasso-Argumedo, J., Curtí-Díaz, S.A.

151

9.6

Asian Citrus Psyllid (ACP) Control: Potential Use of Systemic Insecticides in Citrus Bearing Trees
Yamamoto, P.T., de Miranda, M.P., Felippe, M.R.

152

9.7

Insecticide Resistance and Susceptibility of Uninfected and Candidatus Liberibacter asiaticus-Infected Asian Citrus Psyllid in Florida
Tiwari, S., Rogers, M.E., Stelinski, L.L.

153

9.8

Development of Area-Wide Asian Citrus Psyllid Management Strategies in Texas
Bartels, D.W., Sétamou, M., Ciomperlik, M.A., da Graça, J.V.

154

9.9

Asian Citrus Psyllid Management Strategies for California Citrus Growing Regions
Grafton-Cardwell, E.E., Morse J.G., Taylor, B.J.

155

9.10

Area-Wide Management of Asian Citrus Psyllid in Southwest Florida
Stansly, P.A., Arevalo, H.A., Zekri, M., Hamel, R.


156

9.11

Evaluation of Low Volume Sprayers Used in Citrus Psyllid Control Applications
Hoffmann, C., Fritz, B., Martin, D., Atwood, R., Hurner, T., Ledebuhr, M., Tandy, M., Jackson, J.L., Wisler, G., Polek, M.L.

157

9.12

Identification of Parasitoids and Haplotypes of Tamarixia radiata (Waterston) (Hymenoptera: Eulophidae) from Diaphorina citri in Yucatán, México
González-Hernández, A., Jasso-Argumedo, J., Cruz- García, R., Lozano-Contreras, M., López-Arroyo, J.I., Villanueva-Segura, O.K.

158

9.13

Host Specificity Testing of Tamarixia radiata for the Classical Biological Control of Asian Citrus Psyllid, Diaphorina citri, in California
Pandey, R.R., Hoddle, M.S.

159

9.14

Predators in Non-Commercial Citrus and Preliminary Evaluation of Their Potential Against the Asian Citrus Psyllid in Texas
Pfannenstiel, R.S., Unruh, T.R.

160

9.15

Suitability of Diaphorina citri, Toxoptera citricida, and Aphis spiraecola as Prey for Hippodamia convergens
Qureshi, J.A., Stansly, P.A.

161

9.16

Molecular Analysis of Tamarixia radiata from America Uncovers Extensive Haplotype Variation: Multiple Groups?
de León, J.H., Gastaminza, G.A., Sétamou, M., Cáceres, S., Kanga, L.H.B., Buenahora, J., Parra, J.R., Logarzo, G.A., Stañgret, C.R.W.

162

9.17

Molecular Characterization of a New Entomopathogenic Fungus Isaria poprawskii: A Potential Biocontrol Agent for Diaphorina citri. Development of Isaria-Specific Molecular Markers
de León, J.H., Cabanillas, H.E., Humber, R.A., Murray, K.D., Moran, P., Jones, W.A.

163

9.18

RNAi − Evaluating Injection into Citrus Trees and Grapevine to Target Psyllids and Leafhoppers
Hunter, W.B., Stover, E., Glick, E., Bextine, B.R., Paldi, N.

164

9.19

Using Novel Photonic Fence Technology to Protect Foundation Block and Nursery Stock from Asian Citrus Psyllid
Johanson, E., Patt, J., Mullen, E., Rutschman, P., Pegram, N.

165

9.20

Development of a Diaphorina citri-Specific Molecular Diagnostic Marker for Gut Content Examinations
de León, J.H., Thomas, D.B., Sétamou, M., Hagler, J.R.

166

9.21

Development of a Pathogen Dispenser to Control Asian Citrus Psyllid (ACP) in Residential Citrus
Patt, J., Jackson, M., Dunlap, C., Meikle, W., Adamczyk, J.

167

9.22

Producing New Flush at Will in Citrus to Study ACP-Plant Interactions
Malik, N.S.A., Brockington, J., Pérez, J.L., Mangan, R.L.

168

9.23

Thresholds for Vector Control in Young Citrus Treated for Symptoms of HLB with a Nutrient/SAR Package
Monzó, C., Arevalo, H.A., Stansly, P.A.

169

9.24

Experimental Release Rate Analysis of Volatile Compounds from Wax- Based Dispensers
Neuman, R.D., Mills, D.R., Shelton, A.B.

170

9.25

Vegetation Canopy Airflow Modeling for Airborne Dispersion of DMDS
Shelton, A.B., Neuman, R.D.

171

9.26

Methods and Systems to Deliver Volatile Compounds for Biological Control Strategies
Neuman, R.D., Shelton, A.B., Zee, R.H.
       

172
Session 10: HLB Management − Tim Spann, Moderator

10.1

Trunk Injection of Copper Sulfate Pentahydrate (Magna-Bon) Affects Expression of HLB
Graham, J.H., Irey, M.S., Miele, F.

174

10.2

Chemical Compounds Effective Against the Citrus Huanglongbing Bacterium, Candidatus Liberibacter asiaticus In Planta
Zhang, M.-Q., Powell, C.A., Zhou, L.J., He, Z., Stover, E., Duan, Y.-P.

175

10.3

Regional HLB Management on the Effectiveness of Local Strategies of Inoculum Reduction and Vector Control
Bassanezi, R.B., Yamamoto, P.T., Montesino, L.H., Gottwald, T.R., Amorim, L., Bergamin Filho, A.

176

10.4

The Theory of Managing Huanglongbing with Plant Nutrition and Real World Success in Florida
Spann, T.M., Rouse, R.E., Schumann, A.W.

177

10.5

Nutritional Treatments: Inconsequential Effect on HLB Control and Promote Area-Wide Titer Increase and Disease Spread
Gottwald, T.R., Irey, M.S., Graham, J.H., Wood, B.

178

10.6

Nutritional Approaches for Management of Huanglongbing (Citrus Greening) in China
Xia, Y., Sequeira, R.

179

10.7

First Steps Towards Rescuing Las-Infected Citrus Germplasm
McCollum, G., Stover, E.

180

10.8

Screening Chemical Compounds Against Citrus Huanglongbing Using an Optimized Grafting System from Candidatus Liberibacter asiaticus-Infected Citrus Scions
Zhang, M.-Q., Duan, Y.-P., Powell, C.A.

181

10.9

Discovery of Antimicrobial Small Molecules Against Candidatus Liberibacter asiaticus by Screening Novel SecA Inhibitors Using Structure Based Design
Akula, N., Wang, N.-Y.

182

10.10

The Low Pressure Trunk Injection System: A Technology to Fight Against HLB
Tomas, J.

184

10.11

Does Systemic Acquired Resistance (SAR) Control HLB Disease Development?
Graham, J.H., Myers, M.E., Irey, M.S., Gottwald, T.R.

185

10.12

Use of Growth-Priming Agents to Extend the Growth of HLB-Affected Citrus
He, Z., Zhang, M.-Q., Viana, E., Merlin, T., Duan, Y.-P., Stoffella, P.J., Liptay, A., Powell, C.A.

186

10.13

Evaluation of Foliar Zinc and Manganese for Control of HLB or Associated Symptom Development
Johnson, E.G., Irey, M.S., Gast, T., Bright, D.B., Graham, J.H.

187

10.14

Role of Nutritional and Insecticidal Treatments in Mitigation of HLB: Main Effects and Interactions
Stansly, P.A., Arevalo, H.A., Rouse, R.E.

188

10.15

Use of Horticultural Practices in Citriculture to Survive Huanglongbing
Stuchi, E.S., Girardi, E.A.

189

10.16

Critical Control Point (CCP) Analysis to Build a Model System for Measuring Citrus Propagation Risk Mitigations. II. Sampling and Monitoring
Brown, L.G., Jones, E.M., Hartzog, H.M.

191

10.17

The Need of an Epidemio-Surveillance Network to Prevent Huanglongbing Arrival in the South Mediterranean Basin
Dollet, M., Aubert, B., Imbert, E., Gatineau, F.

192

10.18

Presence of Candidatus Liberibacter asiaticus in Diaphorina citri Kuwayama Collected from Plants for Sale in Florida
Halbert, S.E., Manjunath, K.L., Ramadugu, C., Lee, R.F.

193

10.19

A Model System for Studying Huanglongbing
Manjunath, K.L., Ramadugu, C., Kund, G., Trumble, J., Lee, R.F.
      

194
Session 11: Host Tolerance and Resistance − Fred Gmitter, Moderator

11.1

Incidence of Huanglongbing on Several Sweet Orange Cultivars Budded onto Different Rootstocks at the Citrus Experimental Station (EECB), Bebedouro, São Paulo, Brazil
Stuchi, E.S., Reiff, E.T., Sempionato, O.R., Girardi, E.A., Parolin, L.G., Toledo, D.A.

196

11.2

Host Preference and Suitability of Native North American Rutaceae for the Development of the Asian Citrus Psyllid, Diaphorina citri Kuwayama
Sandoval, J.L., II, Sétamou, M., da Graça, J.V.

197

11.3

Progress Using Transgenic Approaches and Biotechnology-Facilitated Conventional Breeding to Develop Genetic Resistance/Tolerance to HLB in Commercial Citrus
Grosser, J.W., Dutt, M., Shohael, A., Barthe, G.A.

198

11.4

Promoter Regulation of the β-Glucuronidase (GUS) Gene and Antimicrobial Peptide D4E1 in a Citrus Rootstock
Benyon, L.S., Stover, E., Bowman, K., McCollum, G., Niedz, R.

199

11.5

Responses of Transgenic Hamlin Sweet Orange Plants Expressing the attacin A Gene to Candidatus Liberibacter asiaticus Infection
Felipe, R.T.A., Mourão-Filho, F.A., Pereira, E.V., Jr., Lopes, S.A., Sousa, M.C., Mendes, B.M.J.

200

11.6

Screening Antimicrobial Peptides In-Vitro for Use in Developing Huanglongbing and Citrus Canker Resistant Transgenic Citrus
Stover, E., Stange, R., McCollum, G., Jaynes, J.

201

11.7

Response of Citrus Transgenic Plants Expressing STX IA Gene to Candidatus Liberibacter asiaticus
Marques, V.V., Bagio, T.Z., Sugahara, V.H., Vasquez-Souza, G.V., Grange, L., Meneguim, L., Kobayashi, A.K., Bespalhok, J., Pereira, L.F.P., Vieira, L.G.E., Leite, R.P., Jr.

202

11.8

Rootstocks and Pruning Effects on Huanglongbing Incidence on Tahiti Limes in Bebedouro, Northern São Paulo State, Brazil
Stuchi, E.S., Reiff, E.T., Sempionato, O.R., Cantuarias-Avilés, T., Girardi, E.A., Parolin, L.G., Toledo, D.A.

204

11.9

Candidatus Liberibacter asiaticus (CLas) Titer in Field HLB-Exposed Commercial Citrus Cultivars
Stover, E., McCollum, G.

205

11.10

Host Response of Different Citrus Genotypes and Relatives to Candidatus Liberibacter asiaticus Infection
Boscariol-Camargo, R.L., Cristofani-Yaly, M., Malosso, A., Coletta-Filho, H.D., Machado, M.A.

206

11.11

Candidatus Liberibacter asiaticus (CLas) Titer in Poncirus trifoliata and P. trifoliata Hybrids: Inferences on Components of HLB Resistance
Stover, E., Shatters, R.G., Jr., McCollum, G., Hall, D.G., Duan, Y.-P.

207

11.12

The Role of Salicylic Acid and Systemic Acquired Resistance in the Response of Citrus to HLB
Khalaf, A., Febres, V.J., Brlansky, R.H., Gmitter, F.G., Jr., Moore, G.A.

208

11.13

Observations of Citrus Poncirus Hybrid Tolerance to Infection with Candidatus Liberibacter asiaticus
Bowman, K.D., Albrecht, U.

209

11.14

Performance of a Phage Gene in Transgenic Citrus Resistant to Citrus Greening
Jiang, Y., Perazzo, G., Septer, A., Kress, R., Gabriel, D.W.

210

11.15

Genome Sequences of Haploid Clementine Mandarin and Diploid Sweet Orange
Gmitter, F.G., Jr.

211

11.16

Exploring Metabolic Profiles of Plant Tissue with Increased or Decreased Susceptibility
Malik, N.S.A., Pérez, J.L., Brockington, J., Mangan, R.L.
      

212
Take Home Messages: What Can Be Implemented Now or in the Near Future?  
213
 
HLB Pathology Lessons − M. Dewdney / T. Schubert

214
 
Entomology Lessons − L.L. Stelinski / M. Sétamou

219
 
Horticulture Lessons − C. Oswalt / E. Stover

229
               
Addenda

A1

Author Index (also, interactive HTML version)

235

A2

List of Participants

246

A3

Meeting Agenda

265

A4

Session 9 Addendum – Extended Papers 9.24 & 9.25

280




Privacy Policy   |   Copyright © 2018

Disclaimer   |   Contact Us