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NPDN National Meeting 2007

Poster Abstract

29

A comprehensive genome-based diagnostics resource and pipeline for identification of threatening plant pathogens

Authors: JILLIAN M. LANG (1), C. Robin Buell (2), Jan E. Leach (1), Ned Tisserat (1), Tom Powers (3), Andre Levesque (4)

Affiliations: (1) Colorado State University, Fort Collins, CO, USA; (2) The Institute for Genomic Research, Rockville, MD, USA; (3) University of Nebraska, Lincoln, NE, USA; (4) Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada

Diagnosticians are increasingly using molecular markers developed from genomic data to identify threatening plant pathogens. However, widespread use of this technology has been limited by the paucity of genomic data for certain plant pathogens, a lack of a centralized phytopathogen database and the need for bioinformatics training. We have been funded by the USDA to address these limitations by constructing a comprehensive resource to house publicly available genome sequence and annotation data for plant pathogens including viruses, bacteria, fungi, oomycetes, and nematodes and provide search tools to facilitate the use of genomic data by the community. This database and resource will be coupled to 1) an experimental component in which we develop genomic based diagnostic tools for three proof-of-concept pathogens (Xanthomonas, Pythium, and Meloidogyne) and 2) an educational component involving training of plant pathologists and diagnosticians in genomics, bioinformatics, and molecular diagnostic methods. We will present progress on the Comprehensive Phytopathogen Genome Resource (http://cpgr.tigr.org>http://cpgr.tigr.org).

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