NPDN 2007 Meeting - Development of molecular markers for identification of Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola

NPDN National Meeting 2007

Poster Abstract

Development of molecular markers for identification of Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola

Authors: JILLIAN LANG (1), John Hamilton (2), Gena Diaz (1), Marie Anne Van Sluys (3), Ned Tisserat (1), C. Robin Buell (2), Jan Leach (1)

Affiliations: (1) Colorado State University, Fort Collins, CO, USA; (2) The Institute for Genomic Research, Rockville, MD, USA; (3) Universidade de São Paulo, São Paulo, Brazil

Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola cause bacterial blight and leaf streak of rice, respectively. X. oryzae pv. oryzicola has not been reported in the USA and is currently on the USDA select agent list. Although symptomology is distinct, the two pathogens are difficult to differentiate from each other based on cultural or biochemical diagnostic methods. Thus, there is need for molecular-based tools to diagnose and determine geographic origin of these pathovars. Genomic analysis tools available in the Comprehensive Phytopathogen Genome Resource (http://cpgr.tigr.org>http://cpgr.tigr.org) were used to identify over 150 primer pairs with potential to distinguish X. oryzae pv. oryzae from X. oryzae pv. oryzicola. Each primer pair was screened against well-characterized isolates of each pathovar using conventional and multiplex polymerase chain reaction (PCR). Robust primers were tested against a larger number of isolates including geographically diverse strains of both pathovars, several other Xanthomonas species and pathovars, and other bacterial plant pathogens. An extensive set of primers were identified that distinguish X. oryzae species and each pathovar.

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