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© 2013 Plant Management Network.
Accepted for publication 15 October 2013. Published 18 October 2013.


First Report of Geosmithia morbida and Pityophthorus juglandis Causing Thousand Cankers Disease in Butternut


Maryna Serdani, OSU Plant Clinic, Oregon State University, Corvallis, OR 97331; Joshua J. Vlach, Oregon Department of Agriculture, Salem, OR 97301; Kelly L. Wallis, Columbia Ag Research, Hood River, OR 97031; Marcelo Zerillo, Colorado State University, Ft. Collins, CO 80523; Tim McCleary and Jeanne Romero-Severson, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556; and Ned A. Tisserat, Department of Bioagricultural Sciences and Pest Management, Colorado State University, Ft. Collins, CO 80523


Corresponding author: Maryna Serdani.  serdanim@science.oregonstate.edu


Serdani, M., Vlach, J. J., Wallis, K. L., Zerillo, M., McCleary, T., and Tisserat. N. A. 2013. First report of Geosmithia morbida and Pityophthorus juglandis causing thousand cankers disease in butternut. Online. Plant Health Progress doi:10.1094/PHP-2013-1018-01-BR.


Butternut or white walnut (Juglans cinerea L.) is native to the eastern United States and southeastern Canada. The wood is used for paneling, cabinets, and furniture while the nut kernels are sweet, buttery, and popular in baking and candy-making. It is not commonly grown as a landscape tree.

In November 2011, the Oregon State University Plant Clinic received several branches from a butternut tree growing in Lane Co., OR. The large tree displayed sparse foliage and branch dieback (Fig. 1). The branches had small holes and insect galleries associated with girdling cankers (Fig. 2). Eighteen months later, the tree is still standing and the disease does not appear to have progressed since 2011.


 

Fig. 1. Sparse foliage and branch dieback of butternut (Juglans cinerea) with thousand cankers disease in Lane Co., OR.

 

Fig. 2. Geosmithia morbida canker and walnut twig beetle galleries on butternut branch.


Microscopic examination revealed the presence of larvae and adults of the walnut twig beetle (Pityophthorus juglandis) in the galleries (Fig. 3). Geosmithia morbida, the cause of thousand cankers disease (TCD), was consistently isolated from necrotic phloem at the canker margins. The ITS region of the rDNA and the β-tubulin and methionine aminopeptidase genes of two single-spore isolates were amplified and sequenced. Both isolates belonged to a multi-locus haplotype group containing G. morbida isolates collected from southern California walnut (J. californica) and Persian walnut (J. regia) in California, and from black walnut (J. nigra) in Colorado, Oregon, and Tennessee. Pathogenicity studies were not conducted; however, pathogenicity of other isolates of G. morbida to butternut following artificial inoculation has been demonstrated (4). Butternut readily hybridizes with Japanese walnut (J. ailantifolia) and these hybrids, which may closely resemble butternut, have been widely planted. Therefore, the identity of the declining butternut was confirmed using chloroplast-cleaved, amplified polymorphic sequence markers and nuclear microsatellite markers (1).


 

Fig. 3. Larvae of the walnut twig beetle (Pityophthorus juglandis) feeding in the cambium.

 

G. morbida and P. juglandis have previously been reported on black walnut in Oregon as well as in eight other western states and four eastern states (Virginia, Tennessee, North Carolina, and Pennsylvania) (4). The latter four states fall within the native range of butternut. TCD has also been confirmed on Arizona walnut (J. major) in Arizona and New Mexico as well as on English walnut (J. regia), California black walnut (J. californica), and Northern California walnut (J. hindsii) in California (3). Butternut is a relatively slow grower and is already seriously impacted in its native range by another canker disease (butternut canker) caused by the fungus Ophiognomonia clavigignenti-juglandacearum (2). As a result, it was added to the endangered species list in Canada in 2005. Further damage to butternut by TCD in these regions could be very serious. To our knowledge, this is the first report of natural infection of butternut by G. morbida and P. juglandis.


Literature Cited

1. McCleary, T. S., Robichaud, R. L., Nuanes, S., Anagnostakis, S. L., Schlarbaum, S. E., and Romero-Severson, J. 2009. Four cleaved amplified polymorphic sequence (CAPS) markers for the detection of the Juglans ailantifolia chloroplast in putatively native J. cinerea populations. Mol. Ecol. Resour. 9:525-527. doi:10.1111/j.1755-0998.2008.02465.x.

2. Ostry, M. E., and Woeste, K. 2004. Spread of butternut canker in North America, host range, evidence of resistance within butternut populations and conservation genetics. Pages 114-120 in: Black walnut in a new century: Proceedings of the 6th Walnut Council Research Symposium. July 25-28, Lafayette, IN. Gen. Tech. Rep. NC-243. C. H. Michler, P. M. Pijut, J. W. Van Sambeek, M. V. Coggeshall, J. Seifert, K. Woeste, R. Overton, and F. Ponder, Jr., eds. USDA Forest Service, North Central Research Station, St. Paul, MN.

3. Tisserat, N., Cranshaw, W., Leatherman, D., Utley, C., and Alexander, K. 2009. Black walnut mortality in Colorado caused by the walnut twig beetle and thousand cankers disease. Online. Plant Health Progress doi:10.1094/PHP-2009-0811-01-RS.

4. Utley, C., Nguyen, T., Roubtsova, T. V., Coggeshall, M. Ford, T. C., Grauke, L. J., Graves, A. D., Leslie, C. A, McKenna, J., Woeste, K., Yaghmour, M. A., Cranshaw, W., Seybold, S. J., Bostock, R. M., and Tisserat, N. 2013. Susceptibility of walnut and hickory species to Geosmithia morbida. Plant Dis. 97:601-607.