Organizing Committee


Poster Presentations


Poster Presentations


Isolation, purification, and characterization of Phakopsora pachyrhizi isolates

Presenter: David A. Smith(1)

Other authors and affiliations: Chandra Paul(2), Todd A. Steinlage(2), Monte R. Miles(1), Glen L. Hartman(1, 2). (1)USDA-ARS, Urbana, IL 61801, U.S.A.; (2)University of Illinois, Department of Crop Sciences, Urbana, IL 61801, U.S.A.

Soybean rust, caused by Phakopsora pachyrhizi, was first reported in the continental United States in November 2004 and is one of the most important foliar diseases of soybean worldwide. P. pachyrhizi isolates have been obtained from 2006 and 2007 across the United States and are being purified and maintained as either single-spore or bulk isolates. A preliminary set of soybean accessions with characterized genes for resistance have been used to evaluate pathogenic differences among isolates. Depending on the soybean line, some isolates cause an immune or hypersensitive reaction, a reddish brown resistant reaction, or a susceptible tan reaction. Differences in reaction patterns of the isolates indicated variation in virulence. Further isolation, purification, and testing of isolates on a differential set along with DNA typing will define P. pachyrhizi variability across the United States.

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