Organizing Committee


Poster Presentations

Poster Presentations


Mycoparasitism of Phakopsora pachyrhizi by Simplicillium lanosoniveum

Presenter: N. A. Ward

All authors and affiliations: N. A. WARD (1), R. W. Schneider (1), M. Brown (2). (1) Louisiana State University Agricultural Center, Baton Rouge, LA; (2) Louisiana State University, Department of Biological Sciences, LA

Our previous work demonstrated that Simplicillium lanosoniveum colonized sori of Phakopsora pachyrhizi, causal agent of Asian soybean rust. Further, we showed that S. lanosoniveum is a mycophilic fungus that only colonized rust sori. Scanning electron microscopy showed mycophilic attractions of S. lanosoniveum to P. pachyrhizi as hyphae intertwined in sori and around urediniospores. We now document via immunofluorescence microscopy that S. lanosoniveum penetrates and colonizes urediniospores. By staining colonized urediniospores with Calcofluor White and an antiserum specific to P. pachyrhizi, we observed hyphae of the mycoparasite entering and extending through urediniospores. These findings indicate that S. lanosoniveum is a mycoparasite of urediniospores and not simply an inhabitant of sori, which suggests that this organism may be useful as a biological control agent.

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