Organizing Committee


Poster Presentations

Poster Presentations


Monitoring airborne soybean rust urediniospores using passive wind-vane traps during the 2009 season in the South and Central United States

Presenter: J. S. Haudenshield

All authors and affiliations: J. S. HAUDENSHIELD (1), G. L. Hartman (1). (1) USDA-Agricultural Research Service, Urbana, IL

Following the arrival of Phakopsora pachyrhizi in the continental United States in 2004, epidemiological studies have tracked the annual spread of soybean rust by field-scouting for disease and by reporting urediniospores detected in rainwater trap sediments by Q-PCR and in wind-vane adhesive traps by microscopic examination with confirmation by Q-PCR. It has been postulated that airborne translocation of this potential inoculum could be monitored to reveal the location of rust invasion before appreciable crop infection occurs. Wind-vane spore traps were established in June 2009 in the states of Alabama, Arkansas (two), Florida, Illinois (ten), Indiana, Iowa (two), Kentucky, Louisiana (two), Minnesota, Mississippi (four), Missouri (two), Tennessee, and Texas (two). At weekly intervals through October, local cooperators mailed the trapped material to Urbana, Illinois, where total DNA was extracted and any P. pachyrhizi DNA present was quantified by Q-PCR. Rust was eventually detected in every state except Minnesota, but fewer than two spores was detected in five of the northwestern Illinois locations and the central Iowa location. On four instances, a single spore was detected at the northern Iowa location, although no disease was reported in that state. Spores were detected in southeast Arkansas and Tennessee 2 and 4 weeks, respectively, before rust was reported by scouting. High numbers of spores (more than 100) were found after mid-September in central Arkansas, Florida, Louisiana, southeast Missouri, and Tennessee. These results suggest that wind-vane spore trapping coupled with Q-PCR assays may be useful in the tracking, analysis, and forecasting of soybean rust outbreaks.

                                        Back to Poster Presentations