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Poster Presentations


Poster Presentations

Laser microdissected soybean samples infected with Asian soybean rust (Phakopsora pachyrhizi).

Presenter: Benjamin Matthews, USDA-ARS, Soybean Genomics & Improvement Laboratory
Coauthor(s): Vincent Paul Klink1, Margaret MacDonald1, Reid Frederick2 and Katherine Schneider2. 1United States Department of Agriculture, Agricultural Research Service, Soybean Genomics and Improvement Laboratory, Bldg. 006, Beltsville, MD 20705 and 2United States Department of Agriculture, Agricultural Research Service, Foreign Disease-Weed Science Research Unit, Fort Detrick, MD 21702

Asian soybean rust (Phakopsora pachyrhizi) is an emerging threat to the soybean crop in the United States and was identified in the U.S. in November 2004. None of the 18,000 accessions in the USDA-ARS soybean germplasm collection has resistance to all soybean rust isolates. Little is known about the genes and proteins expressed during the life cycle of soybean rust, nor of the genes expressed in susceptible and resistant soybean leaves following rust infection. Therefore, we are using laser microdissection to isolate soybean rust at different stages of the infection process. Also, we are isolating soybean, cv. Komata, cells infected with soybean rust isolate Taiwan 72-1 (susceptible response) and Hawaii 94-1 (resistant response). These samples will be used to construct cDNA libraries for EST analysis to identify genes critical to the life cycle of soybean rust and to the infection process. The samples will also be used to examine gene expression in soybean leaves during rust infection using soybean microarrays to identify differences between the susceptible and resistance response. These data may provide insights into the developmental processes critical to the life cycle of soybean rust, and into the susceptible and resistance responses of soybean. Also, we may obtain valuable clues leading to the development of novel molecular strategies and approaches to combat soybean rust.

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