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Detecting Single Seeds of Small Broomrape (Orobanche minor) with a Polymerase Chain Reaction

N. K. Osterbauer and L. Rehms

November 2002

Research

The seeds of the federally-listed noxious weed and parasitic plant small broomrape are extremely small, making the contamination of fields and seed lots by small broomrape difficult to detect and confirm via conventional methods. Complementary polymerase chain reaction (PCR) primers were developed to amplify small broomrape DNA without also amplifying DNA from other species. The PCR-based assay was sensitive enough to detect a single small broomrape seed.

doi:10.1094/PHP-2002-1111-01-RS

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