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U. S. Horticultural Research Laboratory

University of Florida
Citrus Research &
Education Center


Reaching Beyond Boundaries

Proceedings of the Meeting

Orlando, Florida December 2008


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No. Title and Authors Page

P1 Title Page, Editors, and Copyright 1
P2 Mission and Theme 2
P3 Sponsors and Organizing Committees 3
P4 Conference Overview 4
P5 Index of Presentations 5

Keynote Lectures

K1

Historical perspectives of HLB in Asia
Bernard Aubert

14

K2

Evolution of Citrus, Diaphorina citri, and Liberibacter asiaticus
Andrew Beattie

23

K3

Research and Health Management of Citrus Huanglongbing in Taiwan
Hong-Ji Su

57


Session 1: Current HLB Situation and Concerns Regarding Asian Citrus Greening and the Asian Citrus Psyllid
Peter McClure, Moderator

1.1

Current HLB situation and industry perspective in Asia
Beattie, G.A.C. and Holford, P.

94

1.2

Current HLB situation and citrus producer’s perspective in South America
Clóvis Penalva Monteiro

95

1.3

Current HLB situation and citrus producer’s perspective in US
Jim Snively

 


Session 2: HLB Survey
M. Irey, Moderator

2.1

Current situation of citrus huanglongbing in Cuba
Llauger, R
., Luis, M. Collazo, C., Peña, I., González, C., Batista, L., Teixeira, D., Martins, E., Perdomo, A., Casin, J.C., Peréz, J.L., and Cueto, J.R. and Bové, J.M.

99

2.2

Data trends and results from an HLB laboratory that has processed over 55,000 commercial and research samples over a two year period in Florida.
Irey, M.
, Mai, P., Graham, J., and Johnson, J.

103

2.3

Texas steps up surveys for huanglongbing and the Asian citrus psyllid
daGraca, J. V., Skaria, M., Setamou, M., Kunta, M., Arredondo, M., Salas, B., Parker, P.E.

104

2.4

National plan for detection of HLB in Mexico
Robles, G.P., Sanchez, A.H., Mendez, L.O.

106

2.5

Survey for "Candidatus" Liberibacter species in South Africa
Pietersen, G., Kotzé, A., Phahladira, M.N.B., Schwerdtfeger, M.

110

2.6

Large-scale distribution of Diaphorina citri Kuwayama and citrus huanglongbing in Florida
Halbert, S.E., Manjunath, K., and Brodie, M.W.

112

2.7

Is it possible to replant young groves in an area with endemic HLB – a hierarchical sampling approach to determine infection?
Irey, M., Gottwald, T., Stewart, M., and Chamberlain, H.

116



Session 3: HLB Detection and Diagnostics
J. Hartung, Moderator

3.1

Lessons learned from a comparison and evaluation of multiple HLB testing laboratories employing common and different testing methodologies on a common set of samples
Irey, M., Sieburth, P., Brlansky, R., DaGraça, J., Graham, J., Gottwald, T., Hartung, J., Hilf, M., Kunta, M., Manjunath, K., Lin, H., Ramdugu, C., Roberts, P., Rogers, M., Shatters, R., Sun, X., and Wang, N.

119

3.2

Improved detection of low-titer, non-lethal, seed transmitted Candidatus Liberibacter asiaticus in citrus, periwinkle, and dodder using nested PCR
Zhou, L.-J., Benyon, L. S., Powell, C. A., Gottwald, T., and Duan, Y.-P.

120

3.3

Detection of "Candidatus Liberibacter asiaticus" by cycleave isothermal and chimeric primer-initiated amplification of nucleic acids (Cycleave ICAN)
Urasaki, N., Kawano, S., Mukai, H., Uemori, T., Takeda, O., and Sano, T.

124


3.4

A novel molecular diagnostic tool for improved sensitivity and reliability detection of "Candidatus Liberibacter asiaticus" bacterium associated with huanglongbing (HLB)
Lin, H., Doddapaneni, H., and Civerolo, E. L.

127

3.5

Citrus greening (Huanglongbing) disease in India: Present status and diagnostic efforts
Das, A.K.

129

3.6

Improvement of Candidatus Liberibacter asiaticus diagnosis by nested PCR
Coletta-Filho, H.D., Alves, K.C.S., Carlos, E.F., Pereira, M.A.R., Machado, M.A.

134

3.7

Optimizing qPCR for detection of Candidatus Liberibacter species in plants and psyllid samples
Shatters, R. G., Hunter, W., Hall, D., Niedz, R., Evens, T. J.

135

3.8

Comparison of detection sensitivity of different primer pairs for citrus huanglongbing bacterium
Ding, F., Wang, G., Yi, G., Hong, N., Zhong, Y.

136

3.9

Quantification of viable Candidatus Liberibacter asiaticus in hosts using quantitative PCR with the aid of ethidium monoazide (EMA)
Sagaram, U.S., Trivedi, P., Kim, J-S, Brlansky, R.H., Rogers, M.E., Stelinski, L.L., Oswalt, C., and Wang, N.

138

3.10

PCR for detection of Asian and American strains of Candidatus Liberibacter In citrus, Murraya and Diaphorina from Northwest Argentina
Ramallo, J., Acosta, E., Rojas, A., Stein, B.

139

3.11

Current situation of citrus Huanglongbing associated with "Candidatus Liberibacter asiaticus" in Guangdong, China
Deng, X., Chen, J., Xu, J., Guo, H., Pu, X., Cai, L., and Li, H.

141

3.12

Molecular approach for early detection of Candidatus Liberibacter species in Texas citrus
Bextine, B., Gunawan, S., Hunter, W., and Shatters, R.

142

3.13

Comparison of a starch-based field test for Huanglongbing to results from real-time PCR testing of field samples from symptomatic trees in Florida
Chamberlain, H. L. and M. S. Irey

144


Session 4:
Pathogen Genome Sequencing, Phylogenetics, and Culturing
W. Dawson, Moderator

4.1

Evaluation of potential pathogenicity genes identified by genomic sequencing of Ca. Liberibacter asiaticus – Zhang, S., Reddy, S., and Gabriel, D. W.

146

4.2

Zebra complex and HLB: Seeking a common enemy? Bextine, B. Swatsell, C. and Hunter, W.

149

4.3

Microbiome analysis of HLB pathogen infected citrus using phylochips and 16S rDNA clone library sequencing
Sagaram, S., DeAngelis, K. M., Trivedi, P., Kim, J-S, Anderson, G.L., and Wang, N.

151

4.4

Several Liberibacter and phytoplasma species are individually associated with HLB
Bové, J.M., Teixeira, D.C., Wulff, N.A., Eveillard, S., Saillard, C., Bassanezi, R. B., Lopes, S., Yamamoto, P.T., Ayres, A.J.

152

4.5

Phylogenetic analysis of citrus huanglongbing bacterium based on the sequences of 16S rDNA and 16S / 23S rDNA intergenic regions among isolates in China
Ding, F., Deng, X., Hong, N., Zhong, Y., Wang, G., Yi, G.

156

4.6

Ribosomal RNA operons and genome size of Candidatus Liberibacter americanus, a bacterium associated with citrus huanglongbing in Brazil
Wulff, N.A., Eveillard, S., Foissac, X., Ayres, A.J., and Bové, J.M.

159

4.7

Genome analysis of Candidatus Liberibacter asiaticus reveals unique features for designing HLB control strategies
Duan, Y.-P., Zhou, L.J, Hall, D., Li, W.B., Lin, H., Doddapaneni, H., Liu, L., Vahling, C.M., and Gottwald, T.

165

4.8

Cocultivation of Candidatus Liberibacter asiaticus with actinobacteria from citrus with huanglongbing
Davis, M.J., Mondal, S.N., Chen, H.-Q., Rogers, M.E., Brlansky, R.H.

166

4.9

Development of an Asian citrus psyllid (Diaphorina citri) insect cell line M.W. Lewis and Keyhani, N.O.

167

4.10

Enrichment of Candidatus Liberibacter americanus using an artificial psyllid feeding system
Locali-Fabris, E.C., Coletta-Filho, D., Miranda, M.P., Francisco, C.S., Lopes, J.R.S., Machado, M.A.

168

4.11

Asian citrus psyllid (Diaphorina citri) cell culture methods
Marutani-Hert, M., Hunter, W., Hall, D.G.

169

4.12

Genetic diversity of Candidatus Liberibacter asiaticus and Ca. L. americanus based on sequence variations of their rRNA operon
Zhou, L.J., Powell, C.A., Gottwald, T., Duan, Y.P.

172

4.13

Efficient enrichment of the pathogen DNA from HLB infected host
Chen, C., Yu, Q., Gmitter, F.

176

4.14

Characterization of "Candidatus Phytoplasma asteri" citrus huanglongbing strain in Guangdong, China
Chen, J., Deng, X., Pu, X., Cai, L., and Guo, H.

177

4.15

Visible/near-infrared spectroscopy for discrimination of HLB-infected citrus leaves from healthy leaves
Poole, G., Windham, W., Heitschmidt, G., Park, B., and Gottwald, T.

178


Session 5: Host Pathogen Interaction
J. daGraca, Moderator

5.1

Liberibacter populations in citrus and orange jasmine trees in São Paulo, Brazil
Lopes, S. A., Frare, G. F., Bertolini, E., Wulff, N. A.

182

5.2

The effects of HLB-infection on respiration and development of roots of feroniella rootstock (Feroniella oblata) which showed resistance to HLB bacterium
Ogata, T., Kobori, Y., Kawabe, K., Yonemoto, H., Ohto, Y., Nguyen, T.B., and Nguyen, M.C.

184

5.3

Anatomical evolution of symptoms from infection with the HLB bacterium
Achor, D. S., Chung, K-R., Exteberria, E., Wang, N., and Albrigo, L. G.

185

5.4

Influence of temperature on huanglongbing infection under controlled environment
Gasparoto, M. C. G., Bassanezi, R. B., Lopes, S. A., Frare, G., Martins, E. C., Della Colletta Filho, H., Amorim, L.

186

5.5

Can Ca. Liberibacter asiaticus be transmitted through citrus seed? Hartung, J. S., Halbert, S., Shatters, R.

188

5.6

Biochemical changes after infection with Candidatus Liberibacter asiaticus in citrus
JiangBo, Zhong, Y., Wanghui, Yi, G.

189

5.7

"Candidatus Liberibacter solanacearum" associated with zebra chip of potato is not associated with citrus huanglongbing and is absent in Asian citrus psyllid
Li, W., Abad, J. A., and Levy, L.

190

5.8

Discovery of Candidatus Liberibacter psyllaurous and its insect vector the tomato psyllid (Bactericera cockerelli)
Hansen, A.K., Paine, T.P., Stouthamer, R.

194

5.9

Response of sweet orange (Citrus sinensis) to Candidatus Liberibacter asiaticus infection: microscopy and microarray analyses
Kim, J-S., Sagaram, U.S., Burns, J. K., Li, J-L., and Wang, N.

196

5.10

Asian strains of citrus greening bacterium with genetic and pathogenic variation on pummelo
Miyata, S., Tomimura, K., Furuya, N., Okuda, M., Subandiyah, S., Tsai, C.H., Hung, T.H., Su, H.J., and Iwanami, T.

197

5.11

Detection of Candidatus Liberibacter asiaticus in citrus seedlings germinated from Florida seed
Shatters, R. G.

198

5.12

Assessment of transmission of Liberibacter asiaticus from seed to seedlings of ‘Pineapple’ sweet orange and ‘Carrizo’ citrange
Graham, J. H., Irey, M. S., Dawson, W. O., Hall, D., Duan, Y.

199

5.13

Metabolite changes in HLB orange leaves by GC-MS and other techniques
Cevallos-Cevallos, J.M., Reyes-De-Corcuera, J.I.

201

5.14

Regeneration and chemotherapy of huanglongbing-affected periwinkle
Zhang, M-Q., Duan, Y-P., Powell, C.A.

203

5.15

Differences in secondary metabolites in leaves from trees affected with the greening (HLB) disease
Manthey, J.

207

5.16

Role of Murraya species in the spread of huanglongbing
Ramadugu, C., Lopes, S., Manjunath, K., Halbert, S., Roose, M., and Lee, R.

210

5.17

Identification of a new Liberibacter species associated with diseases of solanaceous plants
Liefting, L., Sutherland, P.W., Ward, L.I., Weir, B.S., Kumarasinghe, L., Quinn, B.D., and Clover, G.R.G.

211


Session 6: Asian Citrus Psyllid (Biology and Genomics)
D. Hall, Moderator

6.1

Gene expression in midgut tissues of Diaphorina citri: application to biology and vector control
Hunter, W., Marutani-Hert, M., Shelby, K., Coudron, T.

214

6.2

Pheromones of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae) elicit behavioral responses from its parasitoid, Tamarixia radiata (Waterston) (Hymenoptera: Eulophidae)
Onagbola, E. O., Rouseff, R. L., Stelinski, L. L.

218

6.3

Effects of freezes on survival of Diaphorina citri
Hall, D. G.

219

6.4

Characterization of electrical penetration graphs of Diaphorina citri Kuwayama (Hemiptera: Psyllidae) in citrus
Bonani, J.P., Fereres, A., Appezzato-da-Gloria, B., Garzo, E.I., Miranda, M.P., Lopes, J.R.S.

222

6.5

Symbionts associated with Diaphorina citri Kuwayama (Hemiptera: Psyllidae) in Brazil and a look into their role
Salvador, I., Cônsoli, F. L.

223

6.6

Endosymbiotic microbiota of Asian citrus psyllid (Diaphorina citri)
Marutani-Hert, M., Hunter, W., Dowd, S., and Hall, D.

224

6.7

FK506-Binding protein from Diaphorina citri (Hemiptera: Psyllidae)
Hunter, W., Shatters, R.

228

6.8

Gene expression in Asian citrus psyllid adults feeding from Florida citrus: Application to biology and vector control
Hunter, W., Shelby, K., Dowd, S., McKenzie, C., Shatters, R.

233

6.9

Asian citrus psyllid, genetic basis of immunity
Marutani-Hert, M., Hunter, W.B., Shelby, K.S.

238

6.10

Effects of host plant on fitness of the Asian citrus psyllid, Diaphorina citri
Tsagkarakis, A. E., and Rogers, M. E.

242

6.11

Development of a potato psyllid (Bactericera cockerelli) cell culture
Bextine, B., Tufts, D., Timmons, C., Hunter, W., Marutani-Hert, M.

244


Session 7: Asian Citrus Psyllid (Ecology and Transmission)

S. Halbert, Moderator

7.1

Acquisition of Candidatus Liberibacter asiaticus by the Asian citrus psyllid, Diaphorina citri, and the potential use of insecticides to prevent pathogen transmission
Rogers, M. E., Brlansky, R. H., Ebert, T. A., Serikawa, R. H., Schumann, R. A., and Stelinski, K. P.

247

7.2

Leaf age influencing acquisition of Candidatus Liberibacter asiaticus by the psyllid vector Diaphorina citriBonani, J.P., Appezzato-da-Gloria, B., Fereres, A., Engels, F.E., Lopes, J.R.S.

249


7.3

Ecological studies on initial invasion of Diaphorina citri into the newly planting citrus fields
Kobori, Y., Nakata, T., Ohto, Y., and Takasu, F.

250

7.4

Spatial distribution of adults of Diaphorina citri Kuwayama (Hemiptera: Psyllidae) in ‘Valencia’ sweet orange trees
Costa, M.G., Felippe, M. R., Garbim, L.F., Carmo-Uehara, A., Yamamoto, P.T., Barbosa, J.C.

251

7.5

Population dynamics of Diaphorina citri in citrus orchards in São Paulo State, Brazil
Yamamoto, P.T., Felippe, M.R., Rugno, G.R., Beloti, V. H., Coelho, J.H.C., Ximenes, N.L., Garbim, L.F., Carmo-Uehara, A.

252

7.6

The seasonal influence of Candidatus Liberibacter asiaticus in the Asian citrus psyllid, Diaphorina citri Kuwayama (Homoptera: Psyllidae) in Okinawa, Japan
Sadoyama, Y., and Takushi, T.

253

7.7

Diurnal patterns in flight activity and effect of light on host finding behavior of the Asian citrus psyllid
Sétamou, M., Sanchez, A., Patt, J., Louzada, E.

255

7.8

Psyllids of citrus orchards in South Texas
Thomas, D.B.

258

7.9

Seasonal occurrence of Candidatus Liberibacter asiaticus in Asian citrus psyllids in Florida
Ebert, T.A., Rogers, M.E., and Brlansky, R. H.

259

7.10

Incidence and population of "Candidatus Liberibacter asiaticus" in Asian citrus psyllids (Diaphorina citri) on citrus plants affected by huanglongbing in Florida
Li, W., Duan, Y.P., Brlansky, R. H., Twieg, E. and Levy, L.

261

7.11

Response of Asian citrus psyllid to aromas emitted by the flushing shoots of their rutaceous host plants in a Y-tube olfactometer
Sétamou, M. and Patt, J. M.

266


Session 8: Economics, Fruit Quality, Crop Loss
L. Baldwin, Moderator

8.1

An update on the effect of HLB on orange juice flavor – 2) Sensory evaluation
Plotto, A., McCollum, G., Baldwin, E., Manthey, J., and Irey, M.

267

8.2

Effect of greening plant disease (huanglongbing) on orange juice flavor and consumer acceptability
Goodrich-Schneider, R., Sims, C., Valim, M., Spann, T., Danyluk M. and Rouseff, R.

269

8.3

Yield reduction caused by huanglongbing in different sweet orange cultivars in São Paulo, Brazil
Bassanezi, R. B., Montesino, L. H., Amorim, L., Gasparoto, M.C.G., Bergamin Filho, A.

270

8.4

The production and price effects of citrus greening in São Paulo and Florida on the world orange juice market
Spreen, T. H., Brown, M.G., Jauregui, C.

274

8.5

The economics of management strategies to mitigate the impact of citrus greening in Florida citrus
Muraro, R.P., Morris, R. A.

275

8.6

An update on the effect of citrus HLB on orange juice flavor 1) Chemical components
Baldwin, E., Manthey, J., Plotto, A., McCollum, G., and Irey, M.

276

8.7

The citrus greening bibliographical database, a new tool for researchers, students and growers
Arevalo, H.A., Snyder, G., and Stansly, P. A.

277


Session 9: International Regulatory Agencies – Regulating HLB

Phil Berger and Wayne Dixon, Moderators

9.0

Select representatives for various countries will present how their National Plant Protection Organizations have addressed or are addressing the challenges of HLB. The emphasis will be on what has worked, what has not, and common international unifying themes.

279


Session 10: Epidemiology
A. Bergamin, Moderator

10.1

Relationship between insecticide sprays and huanglongbing progress in a citrus orchard in São Paulo, Brazil
Bergamin-Filho, A., Gasparoto, M.C.G., Bassanezi, R.B., Amorim, L.

282

10.2

A stochastic spatiotemporal analysis of the contribution of primary versus secondary spread of HLB
Gottwald, T., Irey, M., Bergamin-Filho, A., Bassanezi, R., and Gilligan, C.A.

285

10.3

HLB survival analysis – a spatiotemporal assessment of the threat of an HLB - positive tree to its neighbors
Gottwald, T., Irey, M., and Taylor, E.

291

10.4

Use of mathematical models to inform control of an emerging epidemic
Gilligan, C.A. Cunniffe, N.J., Cook, A.R., DeSimone, R.E. and Gottwald, T.R.

296

10.5

An approach to model the impact of huanglongbing on citrus yield
Bassanezi, R.B., Bassanezi, R.C.

301

10.6

The plantation edge effect of HLB – a geostatistical analysis
Gottwald, T. and Irey, M.

305

10.7

Estimating the spatial distribution of huanglongbing from a sample
Parnell, S., Gottwald, T.R, Irey, M. S., and van den Bosch, F.

309

10.8

Within-tree spatial distribution of Candidatus Liberibacter asiaticus
Gottwald, T., Parnell, S., Taylor, E., Poole, K., Hodge, J., Ford, A., Therrien, L., Mayo, S. and M. Irey

310


Session 11: Psyllid Management Strategies

M. Rogers, Moderator

11.1

Bioecology of Diaphorina citri and Tamarixia radiata: zoning for citrus groves of the State of São Paulo
Torres, M.G. and Parra, J. R. P., (presented by Lopes, J. S.)

315

11.2

Repellent effect of guava odor against adults of citrus psyllid, Diaphorina citri
Zaka, S. M., Zeng, X. N.

316

11.3

Wounding of guava (Psidium guajava L.) leaves produces defensive sulfur volatiles
Rouseff, R. L., Onagbola, E. O., Smoot, J. M., and Stelinski, L.L.

318

11.4

Chemical ecology of Asian citrus psyllid (Diaphorina citri) and potential applications of behavior-modifying chemicals for its management
Stelinski, L.L., Onagbola, O.E., and Rouseff, R.L.

319

11.5

Novel reovirus in Diaphorina citri (Hemiptera: Psyllidae)
Marutani-Hert, M., Hunter, W., Hall, D.

320

11.6

Efficiency of insecticides to control Diaphorina citri, vector of huanglongbing bacteria
Yamamoto, P.T., Felippe, M.R., Beloti, V. H., Rugno, G.R.

324

11.7

Integrated pest management of the Asian citrus psyllid (ACP) in Florida
Stansly, P.A., Qureshi, J.A., and Arevalo, H. A.

326

11.8

Perspectives for biological control of Diaphorina citri (Hemiptera: Psyllidae) in Mexico
Lόpez-Arroyo, J.I., Jasso, J., Reyes, M., Loera-Gallardo, J., Cortez-Mondoaca, E. and Miranda, M.

329

11.9

Investigations of the feasibility for managing the Asian citrus psyllid using Isaria fumosorosea
Avery, P.B., Hunter, W. B., Hall, D. G., Jackson, M. A. Powell, C. A., Rogers, M. E.

331

11.10

Natural enemies of Diaphorina citri Kuwayama in Northwest Mexico
Cortez-Mondaca, E., Lugo-Angulo, N. E., Pérez-Márquez,

332

11.11

Managing Asian citrus psyllid Diaphorina citri with soil and foliar applications of insecticides
Qureshi, J. A., and Stansly, P. A.

333


Session 12: HLB Management Strategies

J. Graham, Moderator

12.1

Factors associated with control of huanglongbing in Sáo Paulo, Brazil: A case study
Belasque, J., Bassanezi, R.B., Yamamoto, P.T., Lopes, S.A., Ayres, A.J., Barbosa, J.C., Tachibana, A., Violante, A.R., Tank, A., Giorgetti, C.L., Di Giorgi, F., Tersi, F. Menezes, G., Dragone, J., Catapani, L.F., Jank, R.H. and Bové, J. M.

337

12.2

Monitoring psyllids for early detection and management of huanglongbing
Manjunath, K.L., Halbert, S.E., Ramadugu, C., and Lee, R.

342

12.3

Occurrence and management strategies for HLB in the State of Paraná, Brazil
Leite, R.P.

343


12.4

Observations gleaned from the geospatially referenced and documented spread of HLB in three commercial groves in Florida and the implications of these observations on scouting and management decisions
Irey, M., Gast, T., Terra, R., and Snively, J.

345

12.5

Effect of strategies of inoculum reduction and vector control on huanglongbing progress
Bassanezi, R. B., Yamamoto, P.T., Gimenes-Fernandes, N., Montesino, L. H., Tersi, F.E.A., Sperandio, P. H., Gottwald, T. R., Bergamin-Filho, A., Amorim, L.

347


12.6

Better management for citrus greening: chemical-uses or guava-interplanting?
Ichinose, K., Bang, D. V., Dien, L. Q.

350

12.7

Imidacloprid-induced systemic acquired resistance (SAR) in Cleopatra mandarin and development of HLB
Graham, J. H., Dawson, W. O., Robertson, C.

352

12.8

Detection of greening in sprouts from citrus tree stumps
Futch, S., Brlansky, R., Irey, M., and Weingarten, S.

353

12.9

Beating huanglongbing – an integrative solution
Hunter, W. B., Peretz, Y., Sela, I., Huet, H., Lapidot, M., Yarden, G.

357

12.10

Flying dragon trifoliate orange rootstock for high density plantings in Sáo Paulo, Brazil
Stuchi, E.S., Silva, S.R., Sempionato, O.R., Reiff, E.T.

358

12.11

Research on the technique of eliminating huanglongbing disease from Tankan
Yun Zhong, Ganjun Yi, Bo Jiang, Nonghui Jiang, Yan Liu

359

12.12

National and international networking to develop solutions for HLB in South America
Franca, F. H., Machado, M. A., Vieira, L.F., Morais, A. M., Astúa, J. F., Lopes, D. B., Druck, S.

360

12.13

Delivery of antibacterial peptides into citrus cultivars for the control of citrus Huanglongbing (HLB; citrus greening)
Gowda, S., Folimonova, S., Robertson, C., Shilts, T., Garnsey, S. M., and Dawson, W. O.

364

12.14

Micro-budded, High Density Citrus Planting: Is There an Opportunity for HLB Control and Financial Returns?
Skaria, M. and Hanagriff, R.

365

12.15

The State of California implements its action plan for Asian Citrus Psyllid
Polek, M. and Luque-Williams, M.J.

369


Session 13: Host Resistance

F. Gmitter, Moderator

13.1

Genetic modification of citrus for resistance against citrus greening: preventing expression of anti-bacterial peptides in the fruit
Gurley, B.

371

13.2

Susceptibility of some local mandarins to a Japanese isolate of Candidatus Liberibacter asiaticus
Iwanami, T., and Miyata, S.

372

13.3

Developing transgenic solutions for HLB resistant citrus at the US Horticulture Research Laboratory
Stover, E., Bowman, K., McCollum, G., Niedz, R.

374

13.4

A newly developed agilent microarray designed for the characterization of citrus responses to pathogens
Moore, G. A., Khalaf, A. A., Febres, V. J., Li, L., and Gmitter, F.

375

13.5

Transgenic approaches to control bacterial diseases of citrus
Astúa-Monge, G., Francischini, F.J.B., Kemper, E., Capella, A., Kitajima, J.P., Ferro, J. A.,da Silva, A.C.R.

376

13.6

Towards the ultimate solution: genetic resistance to HLB in commercial citrus
Grosser, J.W., Dutt, M., Omar, A., Orbovic, V., and Barthe, G. A.

377

13.7

Examination of host responses of different citrus varieties to HLB infection
Folimonova, S. Y., Garnsey, S. M., and Dawson, W. O.

378

13.8

Evaluation of Candidatus Liberibacter spp. in genetically transformed sweet orange ‘Hamlin’ with atacin A gene
Simões, T. S., Boscariol-Camargo, R.L., Mendes, B.M.J., Mourão Filho, F.A.A., Carlos, E.F., Machado, M.A.

379

13.9

Colonization of citrus relatives by Candidatus Liberibacter asiaticus
Boscariol-Camargo, R. L., Simões, T. S., Malosso, A., Carlos, E.F., Coletta Filho, H.D., Machado, M.A.

380

13.10

Occurrences of huanglongbing disease of pomelo (Citrus grandis) in Northern Thailand
Akarapisan, A., Piwkhao, K., Chanbang, Y., Naphrom, D., Santasup, C.

381

13.11

A preliminary survey of HLB survivors found in abandoned citrus groves
Chen, C., Zeng, J., Yi, G., Xiao, Y., Ou, S., Gmitter, F.

386

13.12

Using transgenic NPR1 to enhance systemic acquired resistance (SAR) in citrus
Febres, V. J., Moore, G. A.

387

13.13

Navelina ISA 315 sweet orange: a CVC tolerant cultivar
Stuchi, E.S., Silva, S.R., Coletta-Filho, H.D., Franco, D., Carvalho, S.A., Sempionato, O.R. Donadio, L.C., Alves, K.C.S.

388

13.14

Phloem specific transgene expression of anti-bacterial genes driven by AtSUC2 gene promoter in transgenic citrus plants to develop citrus greening resistance
Omar, A., Dutt, M., Barthe, G., Orbovic, V. and Grosser, J.

390

13.15

Citrus variegated chlorosis damage assessment in six sweet orange cultivars in São Paulo, Brazil
Franco, D., Stuchi, E., Silva, S., Martins, A., Laranjeira, F.

391

13.16

Colonization of Asiatic Citrus Psyllid and Huanglongbing Development on Citrus and Citrus Relatives in Indonesia
Subandiyah, S., Himawan, A., Joko, T., Astuti I.P., Holford, P., Beattie, G.A.C., Krugger, R.

393

13.17

A transgenic approach to the control of citrus greening
Reddy, J.D., and Gabriel, D.W.

394


Session 14: International Citrus Industries: Coping with HLB

Jerry Newlin, Moderator

14.1

Presentation of Ranked International Research Priorities
T. Gottwald and W. Dixon

401

14.2

Florida Citrus Producers Research Advisory Council (FCPRAC)/National Academy of Science (NAS) –Research and Development
Tom Turpen (verbal – no text)



Key Take-Home Messages and a View to the Future: Summaries of Research Sessions   14.3 - 14.10

14.3

HLB status and crop losses
Tim Schubert

409

14.4

Survey, detection, diagnosis
Mike Irey

411

14.5

Pathogen sequencing and culturing
William Dawson

413

14.6

Host pathogen interaction
John daGraça

414

14.7

Psyllid biology and genomics
David Hall

415

14.8

Psyllid management
Michael Rogers

417

14.9

HLB management
Andrew Beattie

419

14.10

Host resistance
Ed Stover

421


Key Take-Home Messages and a View to the Future:
Industry Summaries/Perspectives

14.11

Juliano Ayres (verbal – no text)


14.12

Ray Prewett (verbal – no text)


14.13

Ted Batkin (verbal – no text)


14.14

Peter McClure (verbal – no text)



Key Take-Home Messages and a View to the Future:
Regulatory Summaries/Perspectives

14.15

W. Dixon and P. Berger

425


Key Take-Home Messages and a View to the Future:
Final Wrap up

14.16

Jerry Newlin (verbal – no text)



Addenda

A1

Author Index  (PDF)  (Interactive HTML)

428

A2

List of Participants

436

A3

Meeting Agenda

449

A4

Huanglongbing (HLB) a graft transmissible psyllid-borne citrus disease: Diagnosis and strategies for control in Reunion Island - A case study
(Translation from French of excerpts from the original 1988 Ph.D. Thesis of Bernard Aubert, entitled: Le greening une maladie infectieuse des agrumes d’origine bactérienne transmise par des homoptères psyllidés Stratégies de lutte développées à l’île de la Réunion, Circonstances épidémiologiques en Afrique Asie et modalités d’intervention)

460

A5

The Citrus Huang Lung Bin (Greening) Disease in China
Kung Hsiang LIN and Kung Hsun LIN. South China Agricultural College, Plant Protection Department, South China Agricultural Research Institute, Guangzhou, Guangdong, P. R. C. Document translated from the original Chinese by Kung Hsun LIN and adapted by by B, AUBERT. Original document published in 1956 in Acta Phytopathologica Sinica, Vol. II, Part I, No. I, pp. I-II and Part 2, pp. 14-48.

461

A6

Ranked International Research Priorities: A ranking resulting from a survey of IRCHLB participants
Prepared by T. Gottwald, W. Dixon and E. Taylor

462




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